High affinity vitamin d3 binding proteins
OwnerDavid BAKER, Austin DAY +1inventors
InventorsDavid BAKER, Austin DAY, Per Junior GREISEN
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Abstract
The present disclosure provides isolated polypeptides with vitamin D3 binding activity and methods for their use as detection agents. In another aspect, the invention provides recombinant expression vector comprising an isolated nucleic acid of the invention operably linked to a control sequence. In another aspect, the invention provides recombinant host cells comprising the recombinant expression vector of the invention. In another aspect, the invention provides methods for detecting vitamin D3 or one of its metabolites, such as 25-D3, comprising contacting a sample of interest with a detectable polypeptide of the invention.
Claims16
An isolated polypeptide comprising a polypeptide at least 70% identical over the full length of the amino acid sequence of SEQ ID NO:1.
An isolated polypeptide comprising the amino acid sequence of SEQ ID NO:
- 231 or 232.
Dependencies are inferred from the claim text.