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US 2017/0322211 A1Published application
Pathogen detection by sers nanoparticles
AssigneeColorado School of Mines
InventorsNicholas R. Stambach, Stephanie A. Carr, Christopher R. Cox, Kent J. Voorhees
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Abstract
A phage specific antibody presenting particle, devices and methods related to detection of phage amplification are provided. Specifically, described herein are compositions and methods for detecting Listeria bacteria using an antibody that recognized A511 anti-Listeria phage, wherein the antibody is conjugated to a nanoparticle that can be detected by surface-enhanced Raman scattering (SERS).
Claims20
A Listeria phage-specific antibody-presenting particle comprising:
- a gold metal core;
- a coating comprising at least one Raman active reporter molecule attached to the surface of the metal core;
- a glass coating surrounding said coating;
- and
- a phage-specific antibody attached to the surface of said glass coating.
2 more elements
A method of creating a Listeria phage-specific antibody-presenting nanoparticle the method comprising:
- combining a sulfosuccinimidyl crosslinker with a sulfonic acid buffer to create a crosslinker mixture;
- combining the Listeria phage specific antibody with the crosslinker mixture to create an antibody mixture;
- combining the antibody mixture with the nanoparticle, where in the molar ratio of antibody to nanoparticles is about 350:1 to create a conjugated nanoparticle, and
- thereby creating a Listeria phage-specific antibody-presenting nanoparticle.
1 more element
Dependencies are inferred from the claim text.