In Vitro Oral Biofilm Models of Interdental Spaces and Uses
AssigneeColgate-Palmolive Company
InventorsHans Stettler, Amy Russo RUSSO
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Abstract
Disclosed is an in vitro oral biofilm model of interdental spaces including: a support, and a plurality of substratum pairs attached to the support, wherein an oral biofilm is capable of forming on the substratum pairs, wherein the substratum pairs each include a first member and a second member, and wherein the first member and the second member of each substratum pair are arranged to form a space interval between the first member and second member. Methods for assessing the formation of oral biofilms using the in vitro oral biofilm model and methods for testing agents, such as oral compositions, on biofilm reduction using the present model are also provided.
Claims32
An in vitro oral biofilm model of interdental spaces comprising:
- a support, and
- at least two substratum pairs attached to the support, wherein an oral biofilm is capable of forming on the substratum pairs, wherein the substratum pairs each comprise a first member and a second member, and
- wherein the first member and the second member of each substratum pair are arranged to form a space interval between the first member and second member, and
- wherein the space interval between the first and second member of a first substratum pair is greater than the space interval between the first and second member of a second substratum pair.
(canceled)
A method of forming an oral biofilm, the method comprising:
- providing a substratum pair attached to a support, wherein the substratum pair comprises a first member and a second member, and wherein the first member and the second member are separated to form a space interval between the first member and the second member, and wherein the space interval between the first and second member of a first substratum pair is greater than the space interval between the first and second member of a second substratum pair, and
- providing a liquid growth medium comprising microorganisms capable of oral biofilm production;
- placing the substratum pair into the liquid growth medium;
- and
- incubating the substratum pair in the liquid growth medium to form a biofilm on the substratum pair.
A method for identifying an agent for reducing biofilm in interdental spaces, the method comprising:
- providing at least a first substratum pair and a second substratum pair attached to a support, wherein an oral biofilm is capable of forming on the substratum pairs,
- wherein each substratum pair comprises a first member and a second member, and wherein the first member and the second member are separated to form a space interval between the first member and second member;
- providing a liquid growth medium comprising microorganisms capable of oral biofilm production;
- placing the at least first substratum pair and the second substratum pair into the liquid growth medium comprising microorganisms capable of oral biofilm production;
- incubating the at least first substratum pair and the second substratum pair, thereby forming a biofilm on the at least first substratum pair and the second substratum pair;
- contacting the first substratum pair with a test agent;
- and
- comparing an amount of biofilm on the first substratum pair with an amount on the second substratum pair;
- and
- indicating that the test agent reduces biofilm when the amount of biofilm on the first substratum pair is smaller in comparison to the amount of biofilm on the second substratum pair.
Dependencies are inferred from the claim text.