Biosensor
AssigneeDuke University
InventorsHomme W. Hellinga, Loren L. Looger
Life of this patent
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Abstract
Biosensors are made by attaching covalently or non-covalently at least one reporter group to one or more specific positions of a bacterial periplasmic binding protein (bPBP). Upon binding of ligand to the biosensor, there is a change in the signal transduced by the reporter group.
Claims16
1-22. (canceled)
A method of assaying for glutamate in a sample, comprising contacting a biosensor with said sample under conditions such that said biosensor is able to bind to glutamate present in said sample, wherein said biosensor comprises Escherichia coli (E. coli) glutamate/aspartate binding protein (EBP) comprising a reporter group attached at position 126 of said E. coli EBP, and wherein binding of glutamate in a glutamate-binding pocket of said biosensor causes a change in signaling by said reporter group.
Dependencies are inferred from the claim text.