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US 2017/0322204 A1Published application
Simultaneous detection of biomolecules in biological entities
AssigneesMorphosys AG, Albert-Ludwigs-Universitaet Freiburg
InventorsMarkus Enzelberger, Andreas Boll, Beate Diefenbach-Streiber, Guenter Roth, Felix von Stetten, Fabian Stumpf
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Abstract
The present invention provides methods, immunoassays, kits and devices pertaining to the detection of multiple biomolecules from single cells or other biological entities. It also enables the highly parallel detection of interacting biomolecules from such entities.
Claims39
A method for the detection of two or more biomolecules, said method comprising
- (a) providing a sample comprising a cell comprising said biomolecules,
- (b) spatially separating said cell in a compartment comprising a moiety which is able to bind derivatives of said biomolecules,
- (c) releasing the biomolecules from the cell,
- (d) generating derivatives of said biomolecules,
- (e) allowing the derivatives of said biomolecules to bind to the moiety which is able to bind the derivatives of said biomolecules, and
- (f) detecting or identifying the derivatives of the biomolecules.
3 more elements
Dependencies are inferred from the claim text.