System and method for distinguishing blood components
AssigneeLabthroughput LLC
InventorsDavid ZELMANOVIC, John ROCHE
Life of this patent
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Abstract
A method for measuring concentrations of blood cell components is provided. The method comprises: obtaining a blood sample from a subject, the blood sample comprising red blood cells (RBCs), white blood cells (WBCs), and platelets (PLTs); mixing the blood sample with a non-lysing aqueous solution to form a sample mixture comprising a predetermined tonicity; passing the sample mixture through a flow cell; emitting light towards the flow cell; measuring an amount of light absorbed by the RBCs; measuring an amount of light scattered by WBCs, and PLTs; determining a concentration of each of the RBCs, WBCs, and PLTs present in the sample mixture from the measured amount of light absorbed by the RBCs and scattered by the WBCs and PLTs.
Claims36
A method for measuring concentrations of blood cell components, the method comprising:
- obtaining a blood sample from a subject, the blood sample comprising red blood cells (RBCs), white blood cells (WBCs), and platelets (PLTs);
- mixing the blood sample with a non-lysing aqueous solution to form a sample mixture comprising a predetermined tonicity;
- passing the sample mixture through a flow cell;
- emitting light towards the flow cell;
- measuring an amount of light absorbed by the RBCs;
- measuring an amount of light scattered by WBCs, and PLTs;
- determining a concentration of each of the RBCs, WBCs, and PLTs present in the sample mixture from the measured amount of light absorbed by the RBCs and scattered by the WBCs and PLTs.
A system for measuring concentrations of blood cell components, the system comprising:
- an optical source configured to emit light at a predetermined wavelength;
- a flow cell at least partially disposed within the light emitted by the optical source, the flow cell configured to carry a liquid sample mixture through at least a portion of the light emitted by the optical source;
- a first lens disposed between the optical source and the flow cell;
- a second lens disposed between the first lens and the flow cell;
- and
- a first optical detector configured to detect light emitted by the optical source and absorbed by the sample mixture within a first cone of a first predetermined number of degrees around the incident light.
Dependencies are inferred from the claim text.