Preparation of tyndallized, intact and immunologically active cells of Lactobacillus rhamnosus GG and method for qualitative and quantitative determination thereof
AssigneeProbiotical S.P.A.
InventorGiovanni MOGNA
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Abstract
Tyndallized, intact and immunologically active bacterial cells of Lactobacillus rhamnosus GG (ATCC 53103) is described. A method for preparing the same, as well as an analytical method for the qualitative and quantitative determination of tyndallized, intact and immunologically active bacterial cells of Lactobacillus rhamnosus GG (ATCC 53103) is also described.
Claims15
A method for counting the number of dead cells having an intact cell membrane in a sample of tyndallized bacterial cells of Lactobacillus rhamnosus GG (ATCC 53103), the method comprising:
- preparing a sample containing tyndallized bacterial cells of Lactobacillus rhamnosus GG (ATCC 53103) having a concentration from 105 to 107 cells/ml, by serial dilution;
- adding to said sample a first reagent thiazole orange and a second reagent propidium iodide to obtain a solution;
- adding to said solution a suspension of fluorescent microspheres in sodium azide to obtain a test sample;
- subjecting said test sample comprising live cells and dead cells to flow cytofluorometry;
- and
- counting the dead cells being present in the test sample.
A method for counting the number of tyndallized, dead bacterial cells of Lactobacillus rhamnosus GG (ATCC 53103) having an intact cell membrane in a sample of tyndallized bacterial cells of Lactobacillus rhamnosus GG (ATCC 53103), the method comprising:
- providing the sample of tyndallized bacterial cells of Lactobacillus rhamnosus GG (ATCC 53103);
- and
- applying flow cytofluorometry to the sample.
Dependencies are inferred from the claim text.