Measurement of serum lipoproteins
AssigneeVAP Diagnostics Laboratory, Inc
InventorKrishnaji R. KULKARNI
Life of this patent
Estimating the term…
Abstract
Although a more accurate estimate of a person's risk of cardiovascular disease can be made on the basis of the number of lipoprotein particles per unit volume in the person's blood, current methods all rely on measuring the mass of lipoprotein cholesterol per unit volume. It has been discovered that a rapid and accurate lipoprotein particle count can be obtained by photometry. A method and apparatus are provided for measuring the number of lipoprotein particles in a sample using photometry.
Claims15
A method of measuring lipoprotein particle count in a sample from a subject, the method comprising:
- a. separating a LDL fraction, a HDL fraction and a VLDL fraction in the sample by centrifugation;
- b. introducing the LDL fraction, the HDL fraction and the VLDL fraction into an apparatus for measuring the lipoprotein particle number;
- c. obtaining a photometric measurement in the LDL fraction;
- and
- d. calculating a particle count for the LDL fraction that is a function of the photometric measurement,
- wherein separation of the LDL fraction, the HDL fraction and the VLDL fraction is maintained during steps (b) and (c).
A method of measuring lipoprotein particle count in a sample from a subject, the method comprising:
- a. separating a LDL fraction, a HDL fraction, a Lp(a) fraction, an IDL fraction and a VLDL fraction in the sample by centrifugation;
- b. introducing the LDL fraction, the HDL fraction, the Lp(a) fraction, the IDL fraction and the VLDL fraction into an apparatus for measuring the lipoprotein particle number;
- c. obtaining a photometric measurement in at least one of the LDL fraction, the HDL fraction, the Lp(a) fraction, the IDL fraction and the VLDL fraction;
- and
- d. calculating a particle count for each fraction for which the photometric measurement was obtained
- wherein separation of the LDL fraction, the HDL fraction, the Lp(a) fraction, the IDL fraction and the VLDL fraction is maintained during steps (b) and (c).
Dependencies are inferred from the claim text.