Analytical and diagnostic methods utilizing shigella flexneri apyrase
OwnerPavankumar Asalapuram, Aman Russominventors
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Abstract
A method, comprising the steps of providing a sample containing contaminating nucleoside diphosphates and/or nucleoside triphosphates, such as ATP and/or ATP analogues including deoxyribonucleoside triphosphates; reducing the amount of the contaminating nucleoside diphosphates and/or nucleoside triphosphates in the sample with an apyrase enzyme, wherein said apyrase enzyme is a Shigella flexneri apyrase; and performing an analysis of the sample, wherein said analysis comprises an assay that would have been affected by the contaminating nucleoside diphosphates and/or nucleoside triphosphates had they not been reduced in the reduction step.
Claims29
1-39. (canceled)
A method, comprising:
- providing a sample containing contaminating nucleoside diphosphates nucleoside triphosphates, or both, such as ATP, ATP analogues, or both including deoxyribonucleoside triphosphates;
- reducing the amount of the contaminating nucleoside diphosphates, nucleoside triphosphates, or both in the sample with an apyrase enzyme, wherein said apyrase enzyme is a Shigella flexneri apyrase;
- and
- performing an analysis of the sample, wherein said analysis comprises an assay that would have been affected by the contaminating nucleoside diphosphates, nucleoside triphosphates, or both had they not been reduced.
A Shigella flexneri apyrase comprising an amino-acid sequence having at least 97% sequence identity to SEQ ID NO:
- 4 and having apyrase activity.
A composition comprising a Shigella flexneri apyrase in a buffer having a pH in the range of 6-9, and an ionic strength of at least 300 mM.
Dependencies are inferred from the claim text.