Variant forms of urate oxidase and use thereof
AssigneeHorizon Pharma Rheumatology LLC
InventorsJacob Hartman, Simona Mendelovitz
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Abstract
Genetically modified proteins with uricolytic activity are described. Proteins comprising truncated urate oxidases and methods for producing them, including PEGylated proteins comprising truncated urate oxidase are described.
Claims26
1-37. (canceled)
A nucleic acid molecule comprising a polynucleotide sequence encoding a polypeptide, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:
- 8.
An E. coli comprising a nucleic acid molecule comprising a polynucleotide sequence encoding a polypeptide, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:
- 8.
A method for producing a polypeptide comprising:
- (i) culturing an E. coli comprising a nucleic acid molecule comprising a polynucleotide sequence encoding a polypeptide, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:
- 8, under conditions such that the polynucleotide sequence is expressed by the host cell;
- and
- (ii) isolating the expressed polypeptide.
A method for producing a conjugate comprising a polypeptide and PEG comprising:
- (i) providing a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 8;
- and
- (ii) conjugating the polypeptide to polyethylene glycol (PEG).
Dependencies are inferred from the claim text.