Co-Differentiation Of Monocytes From Allogeneic Donors

A method of producing pro-inflammatory dendritic cells (DCs), comprising the steps of:

• providing a mixture of allogeneic leukocytes, which allogeneic leukocytes have been obtained from at least two different, allogeneic donors;
• isolating allogeneic monocytes from said mixture of allogeneic leukocytes to provide monocyte-enriched allogeneic leukocytes;
• generating non-exhausted immature DCs from said monocyte-enriched allogeneic leukocytes, wherein the generation of non-exhausted immature dendritic cells (DCs) is performed by co-culturing said monocyte-enriched allogeneic leukocytes for 2 to 7 days in aqueous cell culture medium free from non-human serum, said medium being supplemented with interleukin-4 (IL-4) and granulocyte-macrophage colony stimulating factor (GM-CSF);
• and
• activating the non-exhausted immature DCs to obtain pro-inflammatory DCs.