Process for improving the solubility of cell culture media

A process for improving the solubility of a dry cell culture medium by

• a) dissolving a defined amount of dry cell culture medium in a defined amount of solvent whereby a precipitate remains
• b) identifying the amino acid ingredients in the precipitate
• c) preparing at least 3 different new batches of a dry cell culture medium with the composition of the one provided in step a) whereby in all new batches the particle size of all ingredients beside the ones identified in step b) remains unchanged and the amino acid ingredients identified in step b) are admixed to the new batches as follows:
• each amino acid ingredient admixed to a batch has a certain particle size range
• different amino acid ingredients in one batch may have the same or different particle size ranges
• either the particle size ranges of one amino acid ingredient or the combination of particle size ranges of two or more amino acid ingredients are different for each new batch
• d) separately dissolving each batch under the same conditions as in step a).
• e) identifying the batch with the lowest amount of non-dissolved ingredients
• f) optionally repeating steps b) to e) one or several times whereby in step b) the batch with the lowest amount of non-dissolved ingredients (i.e. the one identified in step e) is used.