Multivalent and multispecific gitr-binding fusion proteins
OwnerJohn C. Timmer, Kyle S. Jones +5inventors
InventorsJohn C. Timmer, Kyle S. Jones, Amir S. Razai, Abrahim Hussain, Katelyn M. Willis, Quinn Deveraux, Brendan P. Eckelman
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Abstract
This disclosure generally provides molecules that specifically engage glucocorticoid-induced TNFR-related protein (GITR), a member of the TNF receptor superfamily (TNFRSF). More specifically, the disclosure relates to multivalent and/or multispecific molecules that bind at least GITR.
Claims28
A polypeptide comprising at least two copies of a GITR-binding domain (GITR-BD), wherein the GITR-BD comprises a complementarity determining region 1 (CDR1) comprising the amino acid sequence of SEQ ID NO:
- 138;
- a complementarity determining region 2 (CDR2) comprising the amino acid sequence of SEQ ID NO:
- 141;
- and a complementarity determining region 3 (CDR3) comprising the amino acid sequence of SEQ ID NO:
- 116, and wherein the polypeptide does not comprise a binding domain that binds to an antigen other than GITR.
A tetravalent GITR-targeting molecule comprising two copies of a fusion protein comprising the structure:
- (GITR-BD)-Linker-(GITR-BD)-Linker-Hinge-Fc, wherein
- (a) each GITR-BD is a GITR-binding domain comprising a complementarity determining region 1 (CDR1) comprising the amino acid sequence of SEQ ID NO:
- 138;
- a complementarity determining region 2 (CDR2) comprising the amino acid sequence of SEQ ID NO:
- 141;
- and a complementarity determining region 3 (CDR3) comprising the amino acid sequence of SEQ ID NO:
- 116;
- (b) Linker is a linker polypeptide;
- (c) Hinge is a polypeptide derived from an immunoglobulin hinge region;
- and
- (d) Fc is an immunoglobulin Fc region polypeptide;
- and wherein the tetravalent GITR-targeting molecule does not comprise a binding domain that binds to an antigen other than GITR.
Dependencies are inferred from the claim text.