Mutated immunoglobulin-binding polypeptides
AssigneeGE Healthcare Bio-Sciences AB
InventorsMats ANDER, Goran BAUREN, Tomas BJORKMAN, Gustav RODRIGO
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Abstract
The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamic acid residue at position 15 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of said polypeptide, as well as separation matrices comprising the multimers or polypeptides.
Claims27
1-27. (canceled)
A separation matrix, comprising a plurality of polypeptides or multimers of the polypeptides coupled to a solid support, wherein the polypeptide comprises a mutant of SEQ ID NO 4,
- wherein the amino acid residue at position 23 is a threonine or an alanine,
- wherein the glutamine residue at position 9 has been mutated to an amino acid other than asparagine, glutamine, proline or cysteine, and
- wherein the glutamic acid residue at position 15 has been mutated to an amino acid other than asparagine, proline, cysteine, or glutamine.
(canceled)
Dependencies are inferred from the claim text.