Complexes of cytomegalovirus proteins
AssigneeGlaxosmithkline Biologicals SA
InventorsAndrea CARFI, Yingxia Wen
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Abstract
An isolated human cytomegalovirus (HCMV) membrane protein complex that comprises gH, gL and at least one more HCMV glycoprotein is provided. In some embodiments the complex consists of gH, gL and gO. In other embodiments the complex consists of gH, gL, pUL128, pUL130 and pUL131A. Processes for expressing and purifying such complexes, and subsequent uses of such complexes in immunogenic compositions and vaccines, are also provided.
Claims19
1-27. (canceled)
A composition comprising:
- (A) a single recombinant nucleic acid construct, comprising one or more nucleotide sequences encoding:
- HCMV gH or pentamer-forming fragment thereof, HCMV gL or pentamer-forming fragment thereof, HCMV pUL128 or pentamer-forming fragment thereof, HCMV pUL130 or pentamer-forming fragment thereof, and HCMV pUL131A or pentamer-forming fragment thereof;
- or
- (B) two recombinant nucleic acid constructs, first construct comprising one or more nucleotide sequences encoding gH or pentamer-forming fragment thereof, and HCMV gL or pentamer-forming fragment thereof, the second construct comprising one or more nucleotide sequences encoding pUL128 or pentamer-forming fragment thereof, HCMV pUL130 or pentamer-forming fragment thereof, and HCMV pUL131A or pentamer-forming fragment thereof.
A recombinant nucleic acid construct comprising polynucleotide sequences, wherein the polynucleotide sequences consist of (i) one or more nucleotide sequences encoding gH or pentamer-forming fragment thereof, (ii) one or more nucleotide sequences encoding HCMV gL or pentamer-forming fragment thereof, (iii) one or more nucleotide sequences encoding pUL128 or pentamer-forming fragment thereof (iv) one or more nucleotide sequences encoding HCMV pUL130 or pentamer-forming fragment thereof, and (v) one or more nucleotide sequences encoding HCMV pUL131A or pentamer-forming fragment thereof.
A recombinant nucleic acid construct comprising polynucleotide sequences, wherein the polynucleotide sequences consist of (i) one or more nucleotide sequences encoding gH or pentamer-forming fragment thereof and (ii) one or more nucleotide sequences encoding HCMV gL or pentamer-forming fragment thereof.
A recombinant nucleic acid construct comprising polynucleotide sequences, wherein the polynucleotide sequences consist of (iii) one or more nucleotide sequences encoding pUL128 or pentamer-forming fragment thereof (iv) one or more nucleotide sequences encoding HCMV pUL130 or pentamer-forming fragment thereof, and (v) one or more nucleotide sequences encoding HCMV pUL131A or pentamer-forming fragment thereof.
Dependencies are inferred from the claim text.