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US 2017/0320909 A1Published application
Purification of Biological Molecules
AssigneeEMD Millipore Corporation
InventorsAlex Xenopoulos, Michael Phillips, Wilson Moya, Jad Jaber, Mikhail Kozlov, Ajish Potty, Matthew T. Stone, William Cataldo, Christopher Gillespie
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Abstract
The present invention relates to improved processes and systems for purification of biological molecules, where the processes can be performed in a continuous manner.
Claims30
A process for the purification of a target molecule comprising the steps of:
- a) providing a sample comprising the target molecule and one or more impurities;
- b) adding at least one precipitant to the sample and removing one or more impurities, thereby to recover a clarified sample;
- c) subjecting the clarified sample from step (b) to a bind and elute chromatography step comprising at least two separation units, thereby to obtain an eluate comprising the target molecule;
- and
- d) subjecting the eluate to flow-through purification comprising use of two or more media;
- wherein at least two steps are performed concurrently for at least a portion of their duration and wherein the process comprises only one bind and elute chromatography step.
3 more elements
Dependencies are inferred from the claim text.