Affinity reagents for protein purification

A compound comprising the structure

• wherein the wavy line indicates the point of attachment to a substrate, optionally through a linker;
• wherein R1 is selected from the group consisting of 2-phenylpropylamine, 4-methylbenzylamine, 2-phenylethanamine, benzylamine, 3-phenylpropylamine, 2-methylbutylamine, isoamylamine, isobutylamine, butylamine, propargylamine, ethylamine, 1-amino-3,3-diethoxypropane, 4-amino-2-methyl-1-butanol, 5-amino-1-pentanol, acetamide, 4-(difluoromethoxy)benzylamine, 2-N-propoxyethylamine, 2-methoxyethylamine, 3-isopropoxypropylamine, 3-(methylthio)propylamine, 2-aminoethyl isopropyl ether, 4-bromo-2-fluorobenzylamine, 4-iodobenzylamine, 4-bromobenzylamine, 3-fluoro-5-(trifluoromethyl)benzylamine, 3-bromobenzylamine, 3-chloro-2,6-difluorobenzylamine, 3,4-dichlorobenzylamine, 4-(trifluoromethyl)benzylamine, 2-(3-chlorophenyl)ethylamine, 2-(4-chlorophenyl)ethylamine, 3-chlorobenzylamine, 2-chlorobenzylamine, 3-fluorophenethylamine, 4-fluorophenethylamine, 3-fluorobenzylamine, tetrahydrofurfurylamine, (2,2-dimethyl-[1,3]-dioxolan-4-yl)-methylamine, 3-amino-1-propanol, 2-(2-aminoethoxy)ethanol, 2-thiophenemethylamine, 3-bromo-4-methoxyphenethylamine, 3-furanmethanamine, propanamide, homotaurine, (r)-1-(3-methoxyphenyl)ethylamine, 2-(3,4-dimethoxyphenyl)ethylamine, 3-methoxyphenethylamine, 2-(4-methoxyphenyl)ethylamine, 4-methoxybenzylamine, 2-phenoxyethylamine, 2-methoxybenzylamine, and 3-methoxybenzylamine;
• R2 is selected from the group consisting of 2-phenylpropylamine, 4-methylbenzylamine, 2-phenylethanamine, benzylamine, 3-phenylpropylamine, 2-methylbutylamine, isoamylamine, isobutylamine, butylamine, propargylamine, ethylamine, 1-amino-3,3-diethoxypropane, 4-amino-2-methyl-1-butanol, 5-amino-1-pentanol, acetamide, 1-amino-2-butanol, 4-(difluoromethoxy)benzylamine, 2-N-propoxyethylamine, 2-methoxyethylamine, 3-isopropoxypropylamine, 3-(methylthio)propylamine, 2-aminoethyl isopropyl ether, 4-bromo-2-fluorobenzylamine, 4-iodobenzylamine, 4-bromobenzylamine, 3-fluoro-5-(trifluoromethyl)benzylamine, 3-bromobenzylamine, 3-chloro-2,6-difluorobenzylamine, 3,4-dichlorobenzylamine, 4-(trifluoromethyl)benzylamine, 2-(3-chlorophenyl)ethylamine, 2-(4-chlorophenyl)ethylamine, 3-chlorobenzylamine, 2-chlorobenzylamine, 3-fluorophenethylamine, 4-fluorophenethylamine, 3-fluorobenzylamine, tetrahydrofurfurylamine, (2,2-dimethyl-[1,3]-dioxolan-4-yl)-methylamine, 3-aminopropane-1,2-diol, 3-amino-1-propanol, 2-(2-aminoethoxy)ethanol, 2-(2-methoxyphenyl)ethylamine, 2-thiophenemethylamine, 3-bromo-4-methoxyphenethylamine, 3-furanmethanamine, propanamide, 2-(2-furylmethylthio)ethanamine, homotaurine, (r)-1-(3-methoxyphenyl)ethylamine, 2-(3,4-dimethoxyphenyl)ethylamine, 3-methoxyphenethylamine, 2-(4-methoxyphenyl)ethylamine, 4-methoxybenzylamine, 2-phenoxyethylamine, 2-methoxybenzylamine, 3-methoxybenzylamine, (z)-4-chloro-2-buten-1-amine, 2-(1-cyclohexenyl)ethylamine, 3-methoxypropylamine, 3-ethoxypropylamine, 3-N-propoxypropylamine, 2-ethoxyethylamine, 3-iodobenzylamine, 3-(trifluoromethyl)benzylamine, 4-(4-fluorophenoxy)benzylamine, 3,4-methylenedioxybenzylamine, 2-aminoethyl hydrogen sulfate, aminomethanesulfonic acid, 3,4,5-trimethoxybenzylamine, dl-1-(1-naphthyl)ethylamine, (s)-(−)-1-(4-methylphenyl)ethylamine, (s)-(−)-beta-methylphenethylamine, (r)-(−)-1-aminoindan, (s)-(−)-1-phenylethylamine, (r)-(+)-1-phenylethylamine, 1-phenylbutan-3-amine, d-phenylalaninol, (1r,2s)-1-amino-2-indanol, 2-amino-3,3-dimethylbutane, 1,3-dimethylbutylamine, sec-butylamine, isopropylamine, 2-amino-1-methoxybutane, (s)-2-amino-1-phenylethanol, (s)-(+)-2-amino-1-pentanol, (r)-(−)-2-amino-1-butanol, cyclopentylamine, cyclopropylamine, trans-4-aminocyclohexanol, (1s,2s)-(+)-2-benzyloxycyclohexylamine, (1r,2r)-(−)-2-benzyloxycyclopentylamine, 2-(2-chlorophenyl)ethylamine, 2-fluorophenethylamine, 4-aminotetrahydropyran, dl-homocysteine thiolactone, indan-5-ylamine, 2-fluoroethanamine, (1r,2r)-(−)-2-amino-1-phenyl-1,3-propanediol, 2-amino-3-methyl-3-sulfanylbutanoic acid, (s)-(−)-1-(3-methoxyphenyl)ethylamine, (s)-(−)-1-(4-methoxyphenyl)ethylamine, 9-aminofluorene, 2,4,6-trimethylaniline, 2,4-dimethylaniline, 3,5-dimethyl aniline, 2,5-dimethylaniline, 2-methylaniline, 2,6-diethylaniline, 3-isopropylaniline, 4-isopropylaniline, 2,6-difluoroaniline, 3′,4′-difluoroaniline, 3-chloroaniline, 2-chloroaniline, 2-fluoroaniline, 4-fluoroaniline, 3,4-methylenedioxy-1-aminobenzene, 4-ethoxyaniline, 2-(methylmercapto)aniline, 2-ethoxyaniline, o-anisidine, and 4-amino-1-butanol;
• R3 is selected from the group consisting of 2-phenylpropylamine, 4-methylbenzylamine, 2-phenylethanamine, benzylamine, 3-phenylpropylamine, 2-methylbutylamine, isoamylamine, isobutylamine, butylamine, propargylamine, ethylamine, 4-amino-2-methyl-1-butanol, 5-amino-1-pentanol, acetamide, 1-amino-2-butanol, 4-(difluoromethoxy)benzylamine, 3-(methylthio)propylamine, 4-bromo-2-fluorobenzylamine, 4-bromobenzylamine, 3-fluoro-5-(trifluoromethyl)benzylamine, 3-bromobenzylamine, 3-chloro-2,6-difluorobenzylamine, 3,4-dichlorobenzylamine, 4-(trifluoromethyl)benzylamine, 2-(3-chlorophenyl)ethylamine, 3-chlorobenzylamine, 2-chlorobenzylamine, 3-fluorophenethylamine, 4-fluorophenethylamine, tetrahydrofurfurylamine, (2,2-dimethyl-[1,3]-dioxolan-4-yl)-methylamine, 3-aminopropane-1,2-diol, 3-amino-1-propanol, 2-(2-aminoethoxy)ethanol, 2-(2-methoxyphenyl)ethylamine, 3-bromo-4-methoxyphenethylamine, 3-furanmethanamine, propanamide, 2-(2-furylmethylthio)ethanamine, (r)-1-(3-methoxyphenyl)ethylamine, 2-(3,4-dimethoxyphenyl)ethylamine, 2-(4-methoxyphenyl)ethylamine, 4-methoxybenzylamine, 2-phenoxyethylamine, 2-methoxybenzylamine, 3-methoxybenzylamine, (z)-4-chloro-2-buten-1-amine, 2-(1-cyclohexenyl)ethylamine, 3-methoxypropylamine, 3-ethoxypropylamine, 3-N-propoxypropylamine, 2-ethoxyethylamine, 3-iodobenzylamine, 3-(trifluoromethyl)benzylamine, 4-(4-fluorophenoxy)benzylamine, 3,4-methylenedioxybenzylamine, 2-aminoethyl hydrogen sulfate, aminomethanesulfonic acid, 3,4,5-trimethoxybenzylamine, dl-1-(1-naphthyl)ethylamine, (s)-(−)-1-(4-methylphenyl)ethylamine, 1-phenylbutan-3-amine, d-phenylalaninol, (1r,2s)-1-amino-2-indanol, 2-amino-3,3-dimethylbutane, 1,3-dimethylbutylamine, 2-amino-1-methoxybutane, (s)-2-amino-1-phenylethanol, (s)-(+)-2-amino-1-pentanol, cyclopentylamine, cyclopropylamine, trans-4-aminocyclohexanol, (1s,2s)-(+)-2-benzyloxycyclohexylamine, (1r,2r)-(−)-2-benzyloxycyclopentylamine, 2-(2-chlorophenyl)ethylamine, 2-fluorophenethylamine, 4-aminotetrahydropyran, dl-homocysteine thiolactone, indan-5-ylamine, benzenepropanoic acid, α-(aminomethyl)-benzoic acid, 6-aminocaproic acid, (2s)-4-amino-2-hydroxy-butyric acid, tyramine, 3-(aminomethyl)-, 3-methoxytyramine, 2-fluoroethanamine, phenylalanine, glutamic acid, tyrosine, 4-pyridinepropanoic acid, (1r,2r)-(−)-2-amino-1-phenyl-1,3-propanediol, beta alanine naphthylamide, 2-amino-3-methyl-3-sulfanylbutanoic acid, 1,2,3,4-tetrahydro-1-naphthylamine, (s)-(−)-1-(3-methoxyphenyl)ethylamine, (s)-(−)-1-(4-methoxyphenyl)ethylamine, 3-pyrrolidinecarboxylic acid, pyrrolidine, 9-aminofluorene, 2,6-diethylaniline, 3,4-methylenedioxy-1-aminobenzene, 4-ethoxyaniline, 1h-indol-5-ol, d-phenylalanine, 2-morpholineacetic acid, and 4-amino-1-butanol;
• and
• R4 is selected from the group consisting of 2-phenylpropylamine, 4-methylbenzylamine, 2-phenylethanamine, benzylamine, 3-phenylpropylamine, 2-methylbutylamine, isoamylamine, isobutylamine, butylamine, propargylamine, (z)-4-chloro-2-buten-1-amine, 2-(1-cyclohexenyl)ethylamine, 3-methoxypropylamine, 3-ethoxypropylamine, 3-N-propoxypropylamine, 2-ethoxyethylamine, 3-iodobenzylamine, 3-(trifluoromethyl)benzylamine, 4-(4-fluorophenoxy)benzylamine, 3,4-methylenedioxybenzylamine, ethylamine, 1-amino-3,3-diethoxypropane, 4-amino-2-methyl-1-butanol, 5-amino-1-pentanol, acetamide, 1-amino-2-butanol, 3,4,5-trimethoxybenzylamine, dl-1-(1-naphthyl)ethylamine, (s)-(−)-1-(4-methylphenyl)ethylamine, (s)-(−)-beta-methylphenethylamine, (r)-(−)-1-aminoindan, (s)-(−)-1-phenylethylamine, (r)-(+)-1-phenylethylamine, 1-phenylbutan-3-amine, d-phenylalaninol, (1r,2s)-1-amino-2-indanol, 2-amino-3,3-dimethylbutane, 1,3-dimethylbutylamine, 4-(difluoromethoxy)benzylamine, 2-N-propoxyethylamine, 2-methoxyethylamine, 3-isopropoxypropylamine, 3-(methylthio)propylamine, 2-aminoethyl isopropyl ether, 4-bromo-2-fluorobenzylamine, 4-iodobenzylamine, 4-bromobenzylamine, 3-fluoro-5-(trifluoromethyl)benzylamine, sec-butylamine, isopropylamine, 2-amino-1-methoxybutane, (s)-2-amino-1-phenylethanol, (s)-(+)-2-amino-1-pentanol, (r)-(−)-2-amino-1-butanol, cyclopentylamine, cyclopropylamine, trans-4-aminocyclohexanol, (1s,2s)-(+)-2-benzyloxycyclohexylamine, (1r,2r)-(−)-2-benzyloxycyclopentylamine, 3-bromobenzylamine, 3-chloro-2,6-difluorobenzylamine, 3,4-dichlorobenzylamine, 4-(trifluoromethyl)benzylamine, 2-(3-chlorophenyl)ethylamine, 2-(4-chlorophenyl)ethylamine, 3-chlorobenzylamine, 2-chlorobenzylamine, 3-fluorophenethylamine, 4-fluorophenethylamine, 3-fluorobenzylamine, 2-(2-chlorophenyl)ethylamine, 2-fluorophenethylamine, 4-aminotetrahydropyran, dl-homocysteine thiolactone, indan-5-ylamine, tetrahydrofurfurylamine, (2,2-dimethyl-[1,3]-dioxolan-4-yl)-methylamine, 6-aminocaproic acid, (2s)-4-amino-2-hydroxy-butyric acid, tyramine, 3-methoxytyramine, 2-fluoroethanamine, 3-aminopropane-1,2-diol, 3-amino-1-propanol, 2-(2-aminoethoxy)ethanol, 2-(1h-indol-3-yl)ethanamine (tryptamine), 2-(2-methoxyphenyl)ethylamine, phenylalanine, glutamic acid, tyrosine, 4-pyridinepropanoic acid, 2-amino-3-methyl-3-sulfanylbutanoic acid, 1,2,3,4-tetrahydro-1-naphthylamine, 2-thiophenemethylamine, 3-bromo-4-methoxyphenethylamine, 3-furanmethanamine, propanamide, 2-(2-furylmethylthio)ethanamine, (s)-(−)-1-(3-methoxyphenyl)ethylamine, (s)-(−)-1-(4-methoxyphenyl)ethylamine, 3-pyrrolidinecarboxylic acid, 3-piperidinecarboxylic acid, pyridine, pyrrolidine, 3-pyrrolidinecarboxamide, homotaurine, (r)-1-(3-methoxyphenyl)ethylamine, 2-(3,4-dimethoxyphenyl)ethylamine, 3-methoxyphenethylamine, 2-(4-methoxyphenyl)ethylamine, 4-methoxybenzylamine, 2-phenoxyethylamine, 2-methoxybenzylamine, 3-methoxybenzylamine, and 4-amino-1-butanol, and
• wherein the attachment point between the R1-R4 groups and the structure is through the N of the R1-R4 groups.