Method for manufacturing ciliary margin stem cells
AssigneesSumitomo Chemical Company, Limited, Riken
InventorsAtsushi KUWAHARA, Yoshiki SASAI (deceased)
Life of this patent
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Abstract
The invention provides a method for producing a ciliary marginal zone stem cell induced to differentiate from a pluripotent stem cell, including either the following step (1) or step (2), or both of these steps: (1) a step of floating culturing cells obtained from a cell aggregate containing a ciliary marginal zone-like structure induced to differentiate from pluripotent stem cells, thereby obtaining a retinosphere; and (2) a step of collecting stage specific embryonic antigen-1 positive cells from cells obtained from a cell aggregate containing a ciliary marginal zone-like structure induced to differentiate from pluripotent stem cells.
Claims18
A method for producing a ciliary marginal zone stem cell induced to differentiate from a pluripotent stem cell, comprising either the following step (1) or step (2), or both of these steps:
- (1) a step of floating culturing cells obtained from a cell aggregate comprising a ciliary marginal zone-like structure induced to differentiate from pluripotent stem cells, thereby obtaining a retinosphere;
- and
- (2) a step of collecting stage specific embryonic antigen-1 positive cells from cells obtained from a cell aggregate comprising a ciliary marginal zone-like structure induced to differentiate from pluripotent stem cells.
Dependencies are inferred from the claim text.