Cd8-specific capture agents, compositions, and methods of using and making
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsHeather Dawn Agnew, Bert Tsunyin Lai
Life of this patent
Estimating the term…
Abstract
The present application provides stable peptide-based CD8 capture agents and methods of use as detection agents. The application further provides methods of manufacturing CD8 capture agents.
Claims52
A stable, synthetic capture agent that specifically binds CD8, wherein the capture agent comprises a first ligand having affinity for a first epitope on CD8, a second ligand having affinity for a second epitope on CD8, and a linker covalently connecting the first ligand to the second ligand.
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
A method of producing a synthetic capture agent that specifically binds to a target protein comprising
- a. selecting a first ligand that binds to a first epitope on the target protein,
- b. selecting a second ligand that binds to a second epitope on the target protein,
- c. selecting a linker that has a length that allows the linker to bind both the first ligand and the second ligand when both the first and the second ligands are specifically binding the first and second epitopes, respectively, and
- d. binding the linker to the first and second ligands, thereby producing the synthetic capture agent that specifically binds to the target protein.
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
A method of producing a synthetic capture agent that specifically binds to a target protein comprising
- a. selecting a first ligand that binds to a first synthetic epitope, wherein the first synthetic epitope is at least 90% homologous to a first epitope of the target protein, and wherein at least one amino acid of the first synthetic epitope comprises an azide or an acetylene group;
- b. selecting a second ligand that binds to a second synthetic epitope, wherein the second synthetic epitope is at least 90% homologous to a second epitope of the target protein, and wherein at least one amino acid of the second synthetic epitope comprises an azide or an acetylene group;
- c. selecting a linker that has a length that allows the linker to bind both the first ligand and the second ligand when both the first and the second ligands are specifically binding the first and second epitopes, respectively, and
- d. binding the linker to the first and second ligands, thereby producing the synthetic capture agent that specifically binds to the target protein.
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
Dependencies are inferred from the claim text.