Methods of Selecting T cell Line and Donor Thereof for Adoptive Cellular Therapy
AssigneeMemorial Sloan Kettering Cancer Center
InventorsRichard J. O'Reilly, Ekaterina Doubrovina, Guenther Koehne, Aisha N. Hasan, Susan E. Prockop
Life of this patent
Estimating the term…
Abstract
Disclosed herein are methods of selecting an allogeneic T cell line for therapeutic administration to a patient having or suspected of having a pathogen or cancer. Also disclosed are methods of selecting a donor from whom to derive an allogeneic T cell line for therapeutic administration to a patient having or suspected of having a pathogen or cancer.
Claims106
A method of selecting an allogeneic T cell line for therapeutic administration to a human patient having or suspected of having a pathogen or cancer, comprising:
- selecting a T cell line allogeneic to the patient that recognizes at least one epitope of an antigen of the pathogen or the cancer, using a representation that (i) identifies a plurality of HLA alleles and optionally HLA allele combinations, and (ii) discloses indications of relative activities of T cell lines, each recognizing at least one epitope of an antigen of the pathogen or cancer, and restricted to different ones of the HLA alleles or HLA allele combinations in the plurality;
- wherein in the representation each identified HLA allele or HLA allele combination is associated with the respective indication of relative activity of the T cell line restricted to the HLA allele or HLA allele combination, the relative activities being relative measures of known activity against the pathogen or against the cancer exhibited by the T cell lines;
- wherein
- (A) the T cell line selected has in common with the patient or diseased cells in the patient the HLA allele or HLA allele combination identified by the representation to which the recognition of the T cell line is restricted;
- and
- (B) the HLA allele or HLA allele combination, to which the T cell line selected is restricted, is associated in the representation with an indication of the highest relative activity among the HLA alleles and HLA allele combinations in the representation that are known to be in common with the patient or the diseased cells in the patient and are not otherwise disqualified.
A method of selecting an allogeneic T cell donor from whom to derive an allogeneic T cell line for therapeutic administration to a human patient having or suspected of having a pathogen or cancer, comprising:
- selecting a T cell donor allogeneic to the patient, using a representation that (i) identifies a plurality of HLA alleles and optionally HLA allele combinations, and (ii) discloses indications of relative activities of T cell lines, each recognizing at least one epitope of an antigen of the pathogen or cancer, and restricted to different ones of the HLA alleles or HLA allele combinations in the plurality;
- wherein in the representation each identified HLA allele or HLA allele combination is associated with the respective indication of relative activity of the T cell line restricted to the HLA allele or HLA allele combination, the relative activities being relative measures of known activity against the pathogen or against the cancer exhibited by the T cell lines;
- wherein
- (A) the T cell donor selected has at least one HLA allele or HLA allele combination in common with the patient or diseased cells in the patient;
- and
- (B) one of the at least one HLA allele or HLA allele combination in common with the patient or the diseased cells in the patient is associated in the representation with an indication of the highest relative activity among the HLA alleles and HLA allele combinations in the representation that are known to be in common with the patient or the diseased cells in the patient and are not otherwise disqualified.
A method of selecting an allogeneic T cell donor from whom to derive an allogeneic T cell line for therapeutic administration to a human patient having or suspected of having a pathogen or cancer, comprising:
- selecting a T cell donor allogeneic to the patient who has in common one or more HLA alleles with the patient or diseased cells in the patient, using a representation that (i) identifies a plurality of HLA alleles, and (ii) discloses indications of relative frequencies of generation of T cell lines, each recognizing at least one epitope of an antigen of the pathogen or the cancer, and restricted to different ones of said HLA alleles in the plurality;
- wherein in the representation each identified HLA allele is associated with the respective indication of relative frequency of generation of said T cell lines restricted to the HLA allele, wherein:
- the T cell donor selected has at least one HLA allele in common with the patient or the diseased cells in the patient that is associated in the representation with an indication of higher frequency of generation than HLA alleles of the donor that are not in common with the patient or the diseased cells in the patient.
Dependencies are inferred from the claim text.