Temperature sensitive multivalent bordetella avium vaccines
AssigneeBrigham Young University
InventorsMarcus M. JENSEN, Richard A. ROBISON
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Abstract
The present invention is related to various vaccine compositions against Bordetella avium and methods for preparing and administering the various vaccine compositions. The present invention is related to multivalent vaccines compositions such as bivalent or trivalent vaccines for inoculating turkeys against from Bordetella avium rhinotracheitis or coryza due to Bordetella avium infection as well as methods of preparing and administering the multivalent vaccine compositions.
Claims20
A method of preparing a temperature sensitive Bordetella avium multivalent vaccine, the method comprising the steps of:
- a) isolating a plurality of Bordetella avium isolates from turkey poults, where each of the plurality of Bordetella avium isolates are acquired at different geographic locations;
- b) individually exposing each of the plurality of Bordetella avium isolates to a mutagenic agent;
- c) individually culturing each of the plurality of Bordetella avium isolates after exposure with the mutagenic agent;
- d) isolating a temperature sensitive Bordetella avium strain from each culture of the plurality of Bordetella avium isolates, wherein each of the temperature sensitive Bordetella avium strains at 32° C. stably grows at a rate and at 40° C. grows at a rate slower than the rate at 32° C.;
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- e) combining immunologically effective amounts of each of the temperature sensitive Bordetella avium strains with a pharmaceutically acceptable carrier.
A method of immunizing turkey poults against Bordetella avium, comprising a step of administering to a turkey poult an immunologically effective amount of a temperature sensitive Bordetella avium multivalent vaccine comprising a pharmaceutically acceptable carrier and a plurality of different temperature sensitive Bordetella avium strains that are stably unable to colonize lower tracheas and lungs of the turkey poults.
A temperature sensitive Bordetella avium multivalent vaccine comprising a pharmaceutically acceptable carrier and a plurality of temperature sensitive Bordetella avium strains, wherein at least one of the plurality of temperature sensitive Bordetella avium strains is prepared from a Bordetella avium isolate have a matrix-assisted laser desorption/ionization time of flight spectra that does not cluster with vaccine strain 87.
Dependencies are inferred from the claim text.