Bioactive compositions derivable from platelet concentrates, and methods for preparing and using same
AssigneeCook General Biotechnology LLC
InventorsChristopher Gregory Taylor, Erik John Woods
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Abstract
The present disclosure provides a composition comprising a bioactive fraction derived from a platelet concentrate, methods of making the bioactive fraction, and culture medium supplemented with the bioactive fraction. Preferred bioactive fractions have relatively low fibrinogen concentrations while retaining native growth factors in beneficial amounts and ratios.
Claims30
1-25. (canceled)
A method for preparing a bioactive composition, comprising:
- lysing platelets of a human blood-derived concentrate containing platelets and plasma, to form a lysed platelet preparation;
- forming a gel clot from the lysed platelet preparation by converting fibrinogen to fibrin in the lysed platelet preparation;
- compressing the gel clot to express liquid from the gel clot;
- and
- separating the liquid from solids of the gel clot.
A method for processing a platelet lysate composition, comprising:
- lysing platelets of a human blood-derived concentrate containing platelets and plasma, to form a lysed platelet preparation;
- compressing a gel clot formed by converting fibrinogen to fibrin in a lysed platelet preparation so as to express liquid from the gel clot.
A method for processing a platelet lysate composition, comprising:
- passing a liquid bioactive fraction of a human blood-derived platelet concentrate comprising native components of the platelet concentrate including fibrinogen, albumin, globulin, TGF-β1, EGF, FGF-beta, PDGF-AA, PDGF-BB, SDF-1α, and VEGF, wherein the fibrinogen is present at a level less than 20,000 ng/mL, through at least a first depth filter, so as to remove suspended solids from the liquid bioactive fraction.
33-38. (canceled)
A method for preparing a bioactive composition, the method comprising:
- compressing a clotted platelet lysate material to separate a liquid portion from a solid clotted portion.
Dependencies are inferred from the claim text.