Compositions and methods for modifying a predetermined target nucleic acid sequence
AssigneeTargetgene Biotechnologies Ltd.
InventorsYoel Moshe SHIBOLETH, Dan Michael WEINTHAL
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Abstract
Provided herein are compositions and methods for modifying a predetermined nucleic acid sequence. A programmable nucleoprotein molecular complex containing a polypeptide moiety and a specificity conferring nucleic acid (SCNA) which assembles in-vivo, in a target cell, and is capable of interacting with the predetermined target nucleic acid sequence is provided. The programmable nucleoprotein molecular complex is capable of specifically modifying and/or editing a target site within the target nucleic acid sequence and/or modifying the function of the target nucleic acid sequence.
Claims24
-38. (canceled)
A non-naturally occurring specificity conferring nucleic acid (SCNA) comprising:
- a. a specificity-defining region comprising a nucleotide sequence complementary to a target region of a target DNA molecule, and
- b. a recognition region heterologous to the specificity-defining region and capable of interacting with a polypeptide, wherein the SCNA and the polypeptide is capable of forming a nucleoprotein complex and modifying the target DNA molecule.
A non-naturally occurring specificity conferring nucleic acid (SCNA) comprising:
- a. a specificity-defining region comprising a nucleotide sequence complementary to a target region of a target DNA molecule, and
- b. a recognition region separate from the specificity-defining region and capable of interacting with a polypeptide, wherein the SCNA and the polypeptide is capable of forming a nucleoprotein complex, and wherein the SCNA is capable of guiding the nucleoprotein complex to the target region of the target DNA molecule, and wherein the nucleoprotein complex modifies the target DNA molecule.
A non-naturally occurring specificity conferring nucleic acid (SCNA) comprising:
- a. a specificity-defining region comprising a ribonucleotide sequence complementary to a target region of a target DNA molecule, and
- b. a recognition region separate from the specificity-defining region and comprising a ribonucleotide motif, wherein the ribonucleotide motif is capable of interacting with a polypeptide, wherein the SCNA and the polypeptide is capable of forming a nucleoprotein complex, and wherein the nucleoprotein complex is capable of specifically modifying the target DNA molecule.
Dependencies are inferred from the claim text.