Spray-dried composition comprising beta-galactosidase having transgalactosylating activity in combination with maltodextrin and/or nacl and application of the composition
AssigneeDupont Nutrition Biosciences Aps
InventorsMorten Krog LARSEN, Jacob Flyvholm CRAMER, Bjarne LARSEN, Jens DAMGAARD
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Abstract
A spray-dried composition comprising an enzyme which is a β-galactosidase having transgalactosylating activity and a maltodextrin and/or sodium chloride.
Claims18
A spray-dried composition comprising a polypeptide which is a β-galactosidase having transgalactosylating activity and a maltodextrin.
A spray-dried composition comprising a polypeptide which is a β-galactosidase having transgalactosylating activity and sodium chloride.
The composition of any preceding claim, wherein the polypeptide is an enzyme which is classified in Enzyme Classification (E.C.) 3.2.1.23.
The composition of any preceding claim, wherein the polypeptide has a ratio of transgalactosylating activity:β-galactosidase activity of at least 0.5, at least 1, at least 2, at least 2.5, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 11, or at least 12 at or above a concentration of 3% w/w initial lactose concentration.
The composition of any preceding claim, wherein the polypeptide having a transgalactosylating activity is selected from the group consisting of:
- a. a polypeptide comprising an amino acid sequence having at least 90% sequence identity with SEQ ID NO:
- 1, wherein said polypeptide consists of at most 980 amino acid residues,
- b. a polypeptide comprising an amino acid sequence having at least 97% sequence identity with SEQ ID NO:
- 2, wherein said polypeptide consists of at most 975 amino acid residues,
- c. a polypeptide comprising an amino acid sequence having at least 96.5% sequence identity with SEQ ID NO:
- 3, wherein said polypeptide consists of at most 1300 amino acid residues,
- d. a polypeptide encoded by a polynucleotide that hybridizes under at least low stringency conditions with i) the nucleic acid sequence comprised in SEQ ID NO:
- 9, 10, 11, 12 or 13 encoding the polypeptide of SEQ ID NO:
- 1, 2, 3, 4 or 5;
- or ii) the complementary strand of i),
- e. a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 70% identity to the nucleotide sequence encoding for the polypeptide of SEQ ID NO:
- 1, 2, 3, 4 or 5 or the nucleotide sequence comprised in SEQ ID NO:
- 9, 10, 11, 12 or 13 encoding a mature polypeptide, and
- f. a polypeptide comprising a deletion, insertion and/or conservative substitution of one or more amino acid residues of SEQ ID NO:
- 1, 2, 3, 4 or 5.
The composition according to any preceding claim wherein the polypeptide having transgalactosylating activity is selected from the group consisting of:
- a. a polypeptide comprising an amino acid sequence having at least 96.5% sequence identity with SEQ ID NO:
- 3, wherein said polypeptide consists of at most 1300 amino acid residues,
- b. a polypeptide comprising an amino acid sequence having at least 90% sequence identity with SEQ ID NO:
- 1, wherein said polypeptide consists of at most 980 amino acid residues,
- c. a polypeptide encoded by a polynucleotide that hybridizes under at least low stringency conditions with i) the nucleic acid sequence comprised in SEQ ID NO:
- 9, 10, 11, 12 or 13 encoding the polypeptide of SEQ ID NO:
- 1, 2, 3, 4, or 5;
- or ii) the complementary strand of i),
- d. a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 70% identity to the nucleotide sequence encoding for the polypeptide of SEQ ID NO:
- 1, 2, 3, 4 or 5 or the nucleotide sequence comprised in SEQ ID NO:
- 9, 10, 11, 12 or 13 encoding a mature polypeptide, and
- e. a polypeptide comprising a deletion, insertion and/or conservative substitution of one or more amino acid residues of SEQ ID NO:
- 1, 2, 3, 4 or 5.
The composition according to any preceding claim, wherein the polypeptide having transgalactosylating activity comprises or consists of the amino acid sequence of SEQ ID NO:1, 2, 3, 4 or 5.
The composition according to any preceding claim, wherein the composition contains 0.1 wt % or less polyol.
The composition according to any preceding claim, wherein the composition further contains potato starch.
Dependencies are inferred from the claim text.