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US 2017/0314058 A1Published application
Determination of Ligninases Activities by Nano-structure Initiator Mass Spectrometry
AssigneesThe Regents of the University of California, Sandia Corporation
InventorsTrent R. Northen, Kai Deng
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Abstract
A method for rapid, high throughput screening of the activities of enzymes, especially ligninases and its enzyme cocktails, using nanostructure initiator mass spectrometry (NIMS) surfaces, substrates and methodology.
Claims2
A method for analysis of enzyme activity in a sample, comprising:
- designing NIMS substrates incorporating or simulating H, G, or S lignin units in beta-O-4 linkages, 5-5′ (biphenyl), β-5 (phenylcoumaran), or 4-O-5 (diaryl ether) linkages, and tagging each substrate of the NIMS substrates designed with a unique mass fluorous tag or perfluoronated affinity moiety;
- performing ligninase oxidation reactions on the tagged NIMS substrates designed to generate tagged reaction products using one or more enzymes in the sample;
- identifying and analyzing the tagged reaction products of the ligninase oxidation reactions by nanostructure-initiator mass spectrometry (NIMS);
- and
- performing kinetics study and quantification of ratios of products to substrates of the ligninase oxidation reactions to determine the enzyme activity in the sample.
2 more elements
Dependencies are inferred from the claim text.