US 2017/0306371 A1Published application

Method for producing trehangelin

AssigneesThe Kitasato Institute, Nagase & Co., Ltd.

InventorsSatoshi Omura, Yoko Takahashi, Takuji Nakashima, Yuki Inahashi

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Abstract

An object of the present invention is to provide enzymes and a DNA encoding the enzymes that are involved in biosynthesis of trehangelin which has the potential to be a therapeutic agent for photosensitivity disorder and cosmetics, and to provide a method for producing trehangelin by utilizing the enzymes and a recombinant microorganism. The present invention is directed to a protein having an amino acid sequence of SEQ ID NO: 3, 5, 7 or 9, or a protein having an amino acid sequence of SEQ ID NO: 3, 5, 7 or 9 in which one to several amino acids are deleted, substituted, added and/or inserted or an amino acid sequence having 60% or more homology with the amino acid sequence of SEQ ID NO: 3, 5, 7 or 9 and having an enzyme activity involved in biosynthesis of trehangelin; and a DNA encoding said protein.

Claims20

Dependent claims

A nucleic acid molecule having at least one base sequence selected from the following (i) to (iv):

(i) a base sequence of SEQ ID NO:
2,
a base sequence encoding an amino acid sequence of SEQ ID NO:
3, or
a base sequence encoding an amino acid sequence of SEQ ID NO:
3 in which one to several amino acids are deleted, substituted, added and/or inserted or a base sequence having 90% or more identity with the base sequence of SEQ ID NO:
2, wherein a protein encoded by the base sequence has a 3-ketoacyl-CoA synthase activity;
(ii) a base sequence of SEQ ID NO:
4,
a base sequence encoding an amino acid sequence of SEQ ID NO:
5, or
a base sequence encoding an amino acid sequence of SEQ ID NO:
5 in which one to several amino acids are deleted, substituted, added and/or inserted or a base sequence having 90% or more identity with the base sequence of SEQ ID NO:
4, wherein a protein encoded by the base sequence has a 3-ketoacyl-CoA reductase activity;
(iii) a base sequence of SEQ ID NO:
6,
a base sequence encoding an amino acid sequence of SEQ ID NO:
7, or
a base sequence encoding an amino acid sequence of SEQ ID NO:
7 in which one to several amino acids are deleted, substituted, added and/or inserted or a base sequence having 90% or more identity with the base sequence of SEQ ID NO:
6, wherein a protein encoded by the base sequence has an enoyl-CoA hydratase activity;
and
(iv) a base sequence of SEQ ID NO:
8,
a base sequence encoding an amino acid sequence of SEQ ID NO:
9, or
a base sequence encoding an amino acid sequence of SEQ ID NO:
9 in which one to several amino acids are deleted, substituted, added and/or inserted or a base sequence having 90% or more identity with the base sequence of SEQ ID NO:
8, wherein a protein encoded by the base sequence has an acyltransferase activity.

26 more elements

Dependencies are inferred from the claim text.