Simplified device for nucleic acid amplification adn method for using same

A method for amplifying at least one target nucleic acid, present in a liquid and biological sample of interest, within a disposable device which comprises a solid body, at least one fluid channel connecting an inlet, via which all or part of the sample of interest can be drawn up and/or discharged, and an outlet, which is itself connected to a device for the drawing up and/or discharging of the sample of interest,

• the fluid channel further comprising from the inlet to the outlet:
• a first compartment containing all or part of thermostable constituents required for producing the amplification,
• a means for mixing the thermostable constituents, optionally combined with non-thermostable constituents, with the sample of interest,
• a second compartment containing all or part of the non-thermostable constituents required for producing the amplification,
• and at least one zone intended for heating the sample of interest mixed with the thermostable and non-thermostable amplification constituents, in order to allow the amplification of the target nucleic acid,
• which method consists in:
• (a) drawing up via the inlet all or part of the sample of interest within the device,
• (b) moving the said sample to the first compartment for dissolving the thermostable amplification constituents therein,
• (c) mixing the sample and the thermostable constituents with the mixing means,
• (d) applying a first temperature gradient in order to denature the nucleic acid of interest,
• (e) moving the mixture to the second compartment for dissolving the non-thermostable amplification constituents therein,
• (f) mixing the mixture and non-thermostable constituents with the mixing means, and
• (g) applying at least one second temperature gradient in order to amplify the denatured nucleic acid.