Method for preparing microcarriers, microcarriers, and application thereof
AssigneeFUJIFILM Corporation
InventorCarolina Antonia Francina Maria VAN SPREUWEL-GOOSSENS
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Abstract
Objects of the present invention are to provide a preparation method which makes it possible to obtain porous microcarriers having unique properties and a unique particle size distribution and to provide porous gelatin microcarriers having excellent cell growth properties. According to the present invention, there are provided a method for preparing porous recombinant gelatin microcarriers that includes an emulsification step in which an emulsifier having a specific HLB value is used in a specific amount, and provided porous gelatin microcarriers having specific voids.
Claims20
A method for preparing porous recombinant gelatin microcarriers, comprising:
- (a) a step of generating a first emulsion by mixing a composition containing water, recombinant gelatin, a non-water-soluble liquid, and an emulsifier;
- (b) a step of generating a second emulsion by mixing the first emulsion with a non-water-soluble liquid at a temperature higher than a gelation temperature of gelatin;
- and
- (c) a step of cooling the second emulsion at a temperature at which gelatin coagulates,
- wherein the non-water-soluble liquid used in the step (b) contains an emulsifier having a HLB value of less than 8 in an amount of less than 0.5% by weight.
Porous gelatin microcarriers having voids,
- wherein at least half of the voids are spherical, and/or
- at least half of the voids have a diameter that is −30% to +30% of a mean diameter of the voids.
Dependencies are inferred from the claim text.