Artificially engineered protein hydrogels to mimic nucleoporin selective gating
AssigneeMassachusetts Institute of Technology
InventorsMinkyu Kim, Bradley D. Olsen
Life of this patent
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Abstract
Disclosed are synthetic polypeptides modeled after NspI nucleoporin which are useful for forming hydrogels characterized by selective permeability. The polypeptides and hydrogels formed from them include phenylalanine-glycine (FG) repeats, which are believed to participate in the selectivity of the nuclear pore complex. Also disclosed are filtering devices, drug delivery devices, and methods of separating or selectively filtering macromolecules using the hydrogels.
Claims42
A polypeptide comprising a plurality of contiguous instances of a subsequence represented by PAFSFGAKPDEKKDSDTSK (SEQ ID NO:1).
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A polypeptide comprising a plurality of contiguous instances of a subsequence represented by PAFSFGAKPDEKKDDDTSK (SEQ ID NO:2).
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A polypeptide comprising a core sequence represented by PSFSFGAKSDENKAGATSKPAFSFGAKPEEKKDDNSSKPAFSFGAKSNEDKQDGTAKPA FSFGAKPAEKNNNETSKPAFSFGAKSDEKKDGDASKPAFSFGAKPDENKASATSKPAFS FGAKPEEKKDDNSSKPAFSFGAKSNEDKQDGTAKPAFSFGAKPAEKNNNETSKPAFSFG AKSDEKKDGDASKPAFSFGAKSDEKKDSDSSKPAFSFGTKSNEKKDSGSSKPAFSFGAK PDEKKNDEVSKPAFSFGAKANEKKESDESKSAFSFGSKPTGKEEGDGAKAAISFGAKPE EQKSSDTSK (SEQ ID NO:6);
- and a first leucine zipper domain endblock flanking the N-terminal end or the C-terminal end of the core sequence.
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Dependencies are inferred from the claim text.