Activated bifidobacteria and methods of use thereof
AssigneeEvolve Biosystems Inc
InventorsDavid KYLE, David MILLS, Samara FREEMAN-SHARKY
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Abstract
Some embodiments of the invention include a composition and method for treating dysbiosis in infants. The composition may include a mixture of activated bifidobacteria and a complex oligosaccharide wherein the complex oligosaccharide may be derived from a human or non-human source.
Claims27
A composition comprising activated Bifidobacterium.
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A method of preparing activated Bifidobacterium comprising cultivating Bifidobacterium by incubating the bacteria under conditions whereby at least one of genes Blon_0042, Blon_2334, Blon_2335, Blon_2336, Blon_2337, Blon_2338, Blon_2339, Blon_2344, Blon_2346, Blon_2347, or Blon_2331 is upregulated, or gene Blon_2168 is downregulated, or combinations thereof.
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A method of preparing activated bifidobacteria compositions comprising (a) isolating bifidobacteria from infant feces, (b) purifying the bacteria, (c) inoculating a fermenter with a purified strain of the bacteria, (d) incubating the bacteria in the fermenter in the presence of a mammalian milk oligosaccharide isolated from a mammalian milk source, (e) harvesting the cells, and (f) purifying the bacteria obtained in (e).
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Dependencies are inferred from the claim text.