Compositions, uses, and preparation of platelet lysates
AssigneesEmory University, Children's Healthcare of Atlanta, Inc.
InventorsIan B. Copland, Jacques Galipeau
Life of this patent
Estimating the term…
Abstract
In certain embodiments, this disclosure describes compositions comprising platelet lysates depleted of fibrinogen. In a further embodiment, the composition further comprises a cell culture medium component. This disclosure also provides a method for preparing the composition, comprising the steps of (a) lysing platelets providing a lysate; (b) removing cell debris; and (c) depleting fibrinogen by forming a removable mass by adding a metal salt such as calcium chloride. Furthermore, the disclosure also describes the product produced using said method.
Claims17
-15. (canceled)
A method of making fibrinogen depleted platelet lysate comprising
- (a) lysing platelets providing a platelet lysate;
- (b) removing cell debris;
- and
- (d) depleting fibrinogen from the platelet lysate under conditions wherein the concentration of fibrinogen is less than 50 μg/ml.
Dependencies are inferred from the claim text.