High-purity steviol glycosides
AssigneesPureCircle Sdn Bhd, The Coca-Cola Company
InventorsAvetik MARKOSYAN, Indra PRAKASH, Cyrille JARRIN, Aurelien BADIE, Robert TER HALLE, Cynthia BUNDERS, Daniel AURIOL
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Abstract
Methods of preparing highly purified steviol glycosides, particularly rebaudiosides A, D and M are described. The methods include utilizing recombinant microorganisms for converting various staring compositions to target steviol glycosides. In addition, novel steviol glycosides reb D2, reb M2, and reb I are disclosed, as are methods of preparing the same. The highly purified rebaudiosides are useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.
Claims24
A method for producing highly purified target steviol glycoside composition, comprising the steps of:
- a. providing a starting composition comprising at least one organic compound;
- b. providing a recombinant microorganism containing steviol biosynthesis enzymes, UDP-glycosyltransferases, and optionally UDP-glucose recycling enzymes;
- c. contacting the recombinant microorganism with a medium comprising the starting composition to produce a composition comprising a target steviol glycoside;
- and
- d. separating the target steviol glycoside from the medium to provide a highly purified target steviol glycoside composition.
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14-20. (canceled)
A method for preparing reb I comprising
- a. contacting a starting composition comprising reb A with an enzyme capable of transforming reb A to reb I, UDP-glucose, and optionally UDP-glucose recycling enzymes to produce a composition comprising reb I;
- and
- b. isolating a composition comprising reb I.
The method of claim 22, further comprising purifying the composition comprising reb I to provide reb I having a purity greater than about 95% by weight on an anhydrous basis.
23-26. (canceled)
A method for enhancing the sweetness of a beverage comprising a sweetener comprising the steps of:
- a.) providing a beverage comprising a sweetener;
- and
- b.) adding a sweetness enhancer selected from reb D2, reb M2, reb I or a combination thereof;
- wherein the sweetness enhancer is present in a concentration at or below the sweetness recognition threshold of the sweetness enhancer.
A method for making target steviol glycoside comprising converting starting steviol glycoside to target steviol glycoside using a UDP-glucosyltransferase.
Dependencies are inferred from the claim text.