Chlorella variabilis-derived phosphomannose isomerase gene and application thereof

Abstract

The present invention provides a Chlorella variabilis-derived phosphomannose isomerase gene, herein named ChloPMI. The present invention also provides a prokaryotic expression vector comprising ChloPMI, which can be used for identifying mannose metabolic activity of ChloPMI protein. Further, the present invention provides an expression cassette and a plant expression vector comprising ChloPMI, and a use of the expression cassette and the expression vector in genetic transformation of plants. According to the present invention, the transformation of rice cells is successfully achieved with the plant expression vector constructed from the ChloPMI gene using mannose as a selection agent. According to the present invention, a plant-derived phosphomannose isomerase gene is successfully separated and cloned from Chlorella variabilis. Since the plant-derived phosphomannose isomerase gene is derived from Chlorella variabilis, it is environment-friendly and has no potential hazard to human, which is very beneficial in promoting and applying transgenic products and eliminating any existing doubts on transgenes.

Dependencies are inferred from the claim text.