Genetic loci associated with culture and transformation in maize
AssigneeDow AgroSciences LLC
InventorsTristan E. Coram, Susan M. Jayne, Diaa Alabed, Stephen Foulk, Rajat Aggarwal, Natae Daniels
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Abstract
This invention relates to methods for identifying maize plants that having increased culturability and/or transformability. The methods use molecular markers to identify and to select plants with increased culturability and/or transformability or to identify and deselect plants with decreased culturability and/or transformability. Maize plants generated by the methods of the invention are also a feature of the invention.
Claims12
A method of identifying a maize plant that displays increased culturability and/or transformability, the method comprising:
- a) detecting in germplasm of the maize plant at least nine marker loci, wherein at least one marker locus is located within each chromosomal interval (i)-(ix):
- (i) comprising and flanked by asg62 and magi87535;
- (ii) comprising and flanked by npi386a and gpm174b;
- (iii) comprising and flanked by agrr37b and nfa104;
- (iv) comprising and flanked by umc156a and pco061578;
- (v) comprising and flanked by php20608a and idp6638;
- (vi) comprising and flanked by bnl4.36 and umc1482;
- (vii) comprising and flanked by umc126a and idp8312;
- (viii) comprising and flanked by bnl9.11a and gpm609a;
- and,
- (ix) comprising and flanked by wx1 and bnlg1209;
- and
- b) at least one allele is associated with increased culturability and/or transformability.
A method of identifying a maize plant that displays increased culturability and/or transformability, the method comprising detecting in germplasm of the maize plant a haplotype comprising alleles at one or more marker loci, wherein:
- a) one or more marker loci are located within each chromosomal interval (i)-(ix):
- (i) comprising and flanked by asg62 and magi87535;
- (ii) comprising and flanked by npi386a and gpm174b;
- (iii) comprising and flanked by agrr37b and nfa104;
- (iv) comprising and flanked by umc156a and pco061578;
- (v) comprising and flanked by php20608a and idp6638;
- (vi) comprising and flanked by bnl4.36 and umc1482;
- (vii) comprising and flanked by umc126a and idp8312;
- (viii) comprising and flanked by bnl9.11a and gpm609a;
- and,
- (ix) comprising and flanked by wx1 and bnlg1209;
- and
- b) the haplotype is associated with increased culturability and/or transformability.
A method of marker assisted selected comprising:
- a) obtaining a first maize plant having at least nine marker loci, wherein at least one marker locus is located within each chromosomal interval (i)-(ix):
- (i) comprising and flanked by asg62 and magi87535;
- (ii) comprising and flanked by npi386a and gpm174b;
- (iii) comprising and flanked by agrr37b and nfa104;
- (iv) comprising and flanked by umc156a and pco061578;
- (v) comprising and flanked by php20608a and idp6638;
- (vi) comprising and flanked by bnl4.36 and umc1482;
- (vii) comprising and flanked by umc126a and idp8312;
- (viii) comprising and flanked by bnl9.11a and gpm609a;
- and,
- (ix) comprising and flanked by wx1 and bnlg1209;
- and, the allele of the marker locus is associated with increased culturability and/or transformability;
- b) crossing the first maize plant to a second maize plant;
- c) evaluating the progeny for the at least one allele;
- and
- d) selecting progeny plants that possess the at least one allele.
Dependencies are inferred from the claim text.