Electrochemical tyrosinase enzyme immunoassay biosensor and systems and methods related thereto
AssigneeBoard of Trustees of Michigan State University
InventorsRobert M. WORDEN, Paul S. SATOH
Life of this patent
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Abstract
Devices, assays and methods for detecting analytes in a sample are provided. Biosensor devices include a biosensor interface that includes enzyme-conjugated molecules, antibodies and an enzyme driven redox cycle coupled to an electrically conductive electrode for signal amplification. The biosensor devices are easily adaptable to a variety of assay formats, a variety of target analytes and provide real-time measurements combined with high sensitivity and high specificity for the analyte.
Claims29
A biosensor device comprising:
- an electrically conductive electrode;
- and
- a biosensor interface comprising a primary antibody and one or more redox enzymes, wherein the one or more redox enzymes comprises tyrosinase and propagates a redox cycle by oxidizing a phenolic molecule to a quinone and wherein the electrode is configured to reduce the quinone to generate a reduced form of the phenolic molecule, the primary antibody specific for a target analyte in a sample.
An analyte detecting system comprising:
- an electrically conductive electrode;
- and
- a biosensor interface comprising a primary antibody and one or more redox enzymes, wherein the one or more redox enzymes comprises tyrosinase and propagates a redox cycle by oxidizing a phenolic molecule to a quinone and wherein the electrode is configured to reduce the quinone to generate a reduced form of the phenolic molecule, the primary antibody specific for a target analyte in a sample.
A method for detecting a target analyte in a sample comprising:
- providing a device comprising an electrically conductive electrode and a biosensor interface, the biosensor interface comprising a primary antibody and one or more redox enzymes, wherein the one or more redox enzymes comprises tyrosinase and propagates a redox cycle by oxidizing a phenolic molecule to a quinone and wherein the electrode reduces the quinone to generate a reduced form of the phenolic molecule, the primary antibody specific for a target analyte in a sample;
- and
- contacting the biosensor interface with a sample to be tested for the presence of a target analyte.
Dependencies are inferred from the claim text.