Multianalyte molecular analysis using application-specific random particle arrays
AssigneeBioArray Solutions, Ltd.
InventorsSukanta BANERJEE, Michael SEUL
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Abstract
The present invention provides a method for the generation of novel libraries of encoded magnetic particles from sub-libraries of by the generation of novel sub-libraries of magnetic nanoparticles and encoded particles. The sub-libraries are functionalized on demand are useful in the formation of arrays. The present invention is especially useful for performing multiplexed (parallel) assays for qualitative and/or quantitative analysis of binding interactions of a number of analyte molecules in a sample.
Claims17
A method for the generation of libraries of encoded magnetic particles comprising the steps of (a) generating a first sub-library of magnetic nanoparticles, (b) generating a second sub-library of encoded particles, and (c) bringing into contact said first sub-library with said second sub-library to covalently bind said magnetic nanoparticles to said encoded particles to form said library.
A method for the generation of libraries of encoded magnetic particles comprising the steps of (a) generating a first sub-library of magnetic nanoparticles, (b) generating a second sub-library of encoded particles, (c) providing a coupling site on the magnetic nanoparticles contained in said first sub-library, and (d) bringing into contact said first sub-library with said second sub-library to covalently bind said magnetic nanoparticles to said encoded particles to form said library.
A method for the generation of libraries of encoded magnetic particles comprising the steps of (a) generating a first sub-library of magnetic nanoparticles, (b) generating a second sub-library of encoded particles, (c) providing a coupling site on the magnetic nanoparticles contained in said first sub-library, (d) providing a functional site onto the magnetic nanoparticles contained in said first library, and (d) bringing into contact said first sub-library with said second sub-library to covalently bind said magnetic nanoparticles to said encoded particles to form said library
A library of encoded magnetic particles having a chemical diversity greater than 2.
An array comprising a substrate and a planar assembly of encoded and magnetic particles arranged in a designated area on said substrate.
A method of integrating sample preparation and bioassay using magnetic particles comprising the steps of:
- providing a plurality of magnetic particles comprising at least two different particle populations, each population being distinguishable by a recognition molecule attached thereto, wherein the particles are attached to a chemical characteristic that uniquely identifies a biomolecule of interest that selectively binds to the recognition molecule;
- providing a biological fluid containing biomolecules and allowing said biomolecules to interact with the recognition molecules on the magnetic particles;
- removing the fluid along with unbound components thereof;
- transforming the biomolecules bound to the magnetic particles to produce transformed biomolecules, wherein the transformed biomolecules remain attached to the magnetic particles on which they are synthesized;
- performing a bioassay wherein the binding agents comprise the transformed biomolecules.
A method for performing a bioassay involving integration of sample preparation and parallel molecular interaction assay analysis, comprising
- providing an apparatus comprising at least a sample preparation compartment and an assay compartment, and means for fluidically connecting the sample and the assay compartments;
- providing, in the sample preparation compartment, a biological fluid containing a biomolecule of interest and a plurality of magnetic particles capable of binding to the biomolecule of interest, and allowing the magnetic particles to bind the biomolecules of interest;
- removing the biological fluid along with unbound components of said fluid, while retaining the magnetic particles and the biomolecules bound to said particles;
- releasing said biomolecules from said magnetic particles and transporting said biomolecules from the sample preparation compartment to the assay compartment through the fluidic means;
- and
- performing a bioassay wherein the analyte in the bioassay comprises transported biomolecules of interest.
Dependencies are inferred from the claim text.