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US 2017/0290927 A1Published application
Charge-balanced imaging agents
AssigneesBeth Israel Deaconess Medical Center, Inc., Georgia State University Research Foundation, Inc.
InventorsJohn V. Frangioni, Maged M. Henary
Life of this patent
Estimating the term…
Abstract
The present invention relates to compositions for and methods of optically imaging tissues or cells using imaging agents having desirable in vivo properties that result in improved signal-to-background ratio.
Claims11
1-64. (canceled)
A method of imaging tissue or cells, the method comprising:
- (a) contacting the tissue or cells with an imaging agent comprising a dye;
- (b) irradiating the tissue or cells at a wavelength absorbed by the dye;
- (c) detecting an optical signal from the irradiated tissue or cells, wherein the signal-to-background ratio of the detected optical signal is at least about 1.1, thereby imaging the tissue or cells;
- wherein the dye has the formula:
- or an ester thereof, which permits covalent conjugation of the fluorophore to targeting ligands, wherein:
- E is absent, O or S;
- Q is (CH2)q or a non-ionic oligomeric or polymeric solubilizing moiety;
- R17, R18, R19, and R20 are independently selected from H, an ionic group, a non-ionic oligomeric or polymeric solubilizing moiety, halo, cyano, nitro, C1-4 alkyl, C1-4 alkoxy, and C1-4 haloalkyl;
- R21 is H or N-succinimidyl;
- and
- q is 0, 1, 2, 3, 4, 5, 6, 7, or 8.
8 more elements
A dye, having the formula:
- or an ester thereof, which permits covalent conjugation of the fluorophore to targeting ligands, wherein:
- E is absent, O or S;
- Q is (CH2)q or a non-ionic oligomeric or polymeric solubilizing moiety;
- R17, R18, R19, and R20 are independently selected from H, an ionic group, a non-ionic oligomeric or polymeric solubilizing moiety, halo, cyano, nitro, C1-4 alkyl, C1-4 alkoxy, and C1-4 haloalkyl;
- R21 is H or N-succinimidyl;
- and
- q is 0, 1, 2, 3, 4, 5, 6, 7, or 8.
4 more elements
A method of imaging tissue or cells, the method comprising:
- (a) contacting the tissue or cells with an imaging agent comprising a dye;
- (b) irradiating the tissue or cells at a wavelength absorbed by the dye;
- (c) detecting an optical signal from the irradiated tissue or cells, wherein the signal-to-background ratio of the detected optical signal is at least about 1.1, thereby imaging the tissue or cells;
- wherein the dye has the formula:
- or an ester thereof, which permits covalent conjugation of the fluorophore to targeting ligands.
2 more elements
A dye, having the formula:
- or an ester thereof, which permits covalent conjugation of the fluorophore to targeting ligands.
Dependencies are inferred from the claim text.