Methods and compositions for intra-nasal immunization with recombinant mva encoding flagellin

An immunogenic composition comprising a recombinant modified vaccinia virus Ankara (MVA) comprising a nucleic acid sequence encoding a flagellin and a nucleic acid sequence encoding a heterologous disease-associated antigen, wherein the immunogenic composition induces increased T-cell and/or B-cell immune responses specific for the heterologous disease-associated antigen when administered to a subject as compared to T-cell and/or B-cell immune responses specific for the heterologous disease-associated antigen induced by administration of a recombinant MVA comprising a nucleic acid sequence encoding a heterologous disease-associated antigen but not a nucleic acid sequence encoding a flagellin.

A method of inducing an antigen-specific immune response to a disease-associated antigen in a subject comprising administering to the subject an immunogenic composition comprising a nucleic acid sequence encoding a flagellin and a nucleic acid sequence encoding a heterologous disease-associated antigen, wherein the immunogenic composition induces increased T-cell and/or B-cell immune responses specific for the heterologous disease-associated antigen as compared to T-cell and/or B-cell immune responses specific for the heterologous disease-associated antigen induced by administration of a recombinant MVA comprising a nucleic acid sequence encoding a heterologous disease-associated antigen but not a nucleic acid sequence encoding a flagellin.