Global gene regulators (ggr) as vaccine candidates against paratuberculosis
AssigneeWisconsin Alumni Research Foundation
InventorAdel M. Talaat
Life of this patent
Estimating the term…
Abstract
Described herein is a mycobacterium mutant, comprising at least one mutation in at least one gene sequence encoding global gene regulators (GGRs) selected from the group consisting of sigH, sigL, sigE, ECF-1, and mixtures thereof, wherein the GGR gene is at least partially inactivated. Described herein also is a vaccine based on the mutant and a method of differentiating between subjects that have been infected with mycobacterium and subjects that have not been infected with mycobacterium or have been vaccinated with a mycobacterium vaccine.
Claims31
A mycobacterium mutant, comprising at least one mutation in at least one gene sequence encoding global gene regulators (GGRs) selected from the group consisting of sigH, sigL, sigE, ECF-1, and mixtures thereof, wherein the GGR gene is at least partially inactivated.
A method of differentiating between subjects that have been infected with mycobacterium and subjects that have not been infected with mycobacterium or have been vaccinated with a mycobacterium vaccine, the method comprising the steps of
- (a) identifying a sequence that is specific to a mycobacterium mutant and is not found in the wild-type mycobacterium strain;
- and
- (b) detecting the presence of the sequence in the subject.
A method of differentiating between subjects that have been infected with mycobacterium and subjects that have not been infected with mycobacterium or have been vaccinated with a mycobacterium vaccine, the method comprising the steps of
- (a) identifying a sequence that is specific to a wild-type mycobacterium strain and is not found in the mycobacterium mutant;
- and
- (b) detecting the presence or the quantity of the sequence in the subject.
Dependencies are inferred from the claim text.