Compositions and methods for long acting proteins
AssigneeNovartis AG
InventorsJoy GHOSH, Michael ROGUSKA, Andrew Anh NGUYEN, Thomas PIETZONKA, Stephen Hedrick POOR, Matthias MACHACEK, Chad Eric BIGELOW
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Abstract
The present invention is based, in part, on the discovery of peptide tags that increase the half-life and/or mean residence time of proteins or nucleic acids in the eye. In certain aspects the invention peptide tags increase the half-life and/or mean residence time of antibodies and antigen binding fragments, therapeutic proteins, protein receptors, DARPins and/or aptamers in the eye.
Claims44
1) A peptide tag that binds hyaluronan (HA), wherein said peptide tag comprises a sequence a) selected from the group consisting of SEQ ID NO:
- 33, SEQ ID NO:
- 34, SEQ ID NO:
- 35, SEQ ID NO:
- 36, SEQ ID NO:
- 352, SEQ ID NO:
- 353, SEQ ID NO:
- 354, and SEQ ID NO:
- 355;
- or b) 95 consecutive amino acids of a sequence selected from the group consisting of SEQ ID NO:
- 33, SEQ ID NO:
- 34, SEQ ID NO:
- 35, SEQ ID NO:
- 36, SEQ ID NO:
- 352, SEQ ID NO:
- 353, SEQ ID NO:
- 354, and SEQ ID NO:
- 355.
44) A method of increasing the ocular half-life of a protein or nucleic acid comprising linking said protein or nucleic acid to a peptide tag a) selected from the group consisting of SEQ ID NO:
- 33, SEQ ID NO:
- 34, SEQ ID NO:
- 35, SEQ ID NO:
- 36, SEQ ID NO:
- 352, SEQ ID NO:
- 353, SEQ ID NO:
- 354, and SEQ ID NO:
- 355;
- or b) 95 consecutive amino acids of a sequence selected from the group consisting of SEQ ID NO:
- 33, SEQ ID NO:
- 34, SEQ ID NO:
- 35, SEQ ID NO:
- 36, SEQ ID NO:
- 352, SEQ ID NO:
- 353, SEQ ID NO:
- 354, and SEQ ID NO:
- 355.
Dependencies are inferred from the claim text.