Method of making a personalized bone graft
AssigneeThe Trustees of Columbia University in the City of New York
InventorsGordana Vunjak-Novakovic, Warren Grayson, Keith Yeager
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Abstract
An anatomically-shaped, human bone graft may be cultivated ex vivo using a bioreactor capable of perfusing large complex porous scaffolds. Scaffolds derived from image-based modeling of a target are seeded with human mesenchymal stem cells and cultivated. A bioreactor configured to house complex three-dimensional scaffold geometries provides controlled flow for perfusion of the cells. Dense uniform cellular growth can be attained throughout the entire scaffold as a result of the medium perfusion. In an embodiment, the bioreactor has a mold into which perfusion medium is pumped under pressure and multiple ports through which the medium exits the mold.
Claims3
A tissue engineering system comprising:
- a machining device configured to machine a three-dimensional vessel with an internal surface closely following a shape of a target anatomy of a patient;
- a bioreactor having a recess configured to receive said vessel and outlet ports configured to accept at least one lumen and permit flow communication between the at least one lumen and an internal volume defined by the internal surface of said vessel;
- and
- a flow mechanism configured to remove a perfusate from the at least one lumen and return it to the internal volume of the vessel.
Dependencies are inferred from the claim text.