High rebaudioside-c plant varietal and compositions extracted therefrom with high rebaudioside-c and total steviol glycoside content
AssigneeGlg Life Tech Corporation
InventorsQibin WANG, Luke Yong ZHANG, Kevin Cunbiao LI
Life of this patent
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Abstract
A new stevia variety is characterized by remarkably high levels of Rebaudioside C (RC), and developed by the use of non-GMO selective breeding technologies and wherein such variety is uniquely identified by RAPD gene analysis, and comprises from about 3-8% by weight RC in the leaf (leaf content) and from about 10-13% total steviol glycosides content (TSG) in in leaf (leaf content), both of which are exceptionally high.
Claims20
A Stevia rebaudiana plant that comprises a Rebaudioside C leaf content of from 3-8% by weight, and a total steviol glycoside leaf content of at least 10-13% by weight and contains three bands, one between each of i) 250 bp-500 bp, ii) 500 bp-750 bp, and about iii) 1000 bp, and which contains no specific band at 2000 bp, when analyzed by Random Amplified Polymorphic DNA (RAPD) using the primers of
- SEQ ID NO:
- 1
- (5′-TTTGGTGACGGTGCGG-3′),
- SEQ ID NO:
- 2
- (5′-TGGGGCCAACCCAAGTC-3′),
- SEQ ID NO:
- 3
- (5′-GGCCTGCAGCTCTTCT-3′)
- and
- SEQ ID NO:
- 4
- (5′-GCGTCCCCAACTCGATC-3′).
A method for breeding Stevia rebaudiana with a high content of Rebaudioside C, which comprises the following steps:
- selecting a plant with a Rebaudioside C content in the leaf of at least 3% by weight (”core plant“), asexually reproducing the core plant to produce parent plants, hybridizing the parent plants to produce F1 generation seeds, stabilizing the traits of the F1 generation (namely, in the leaves of the F1 generation, producing a Rebaudioside C content of at least 3-8% by weight, and a total steviol content of at least 10-13%) and thereafter producing F2 generation seeds by a backcross method.
The composition of claim 18, wherein the composition comprises a natural sweetener composition from 4-8% by weight Rebaudioside C, from 2-5% by weight Rebaudioside A from 0.5-3% by weight Stevioside.
The composition of claim 18, wherein the composition comprises a natural sweetener composition comprising from 5-8% by weight Rebaudioside C, from 3-4% by weight Rebaudioside A, from 1-2% by weight STV.
The composition of claim 18, further comprising a natural flavour composition.
The composition of claim 18, wherein the composition further comprises foods, beverages, nutraceuticals, functional foods, medicinal formulations, cosmetics, health products, and/or condiments and seasonings.
The composition of claim 18, wherein the composition further comprises a steviol glycoside composition (based on dry leaf weight):
- from 4-8% by weight Rebaudioside C
- from 2-5% by weight Rebaudioside A
- from 0.5-3% by weight Stevioside
The composition of claim 18, wherein the composition further comprises a steviol glycoside composition (based on dry leaf weight):
- from 5-8% by weight Rebaudioside C
- from 3-4% by weight Rebaudioside A
- from 1-2% by weight STV
Dependencies are inferred from the claim text.