# US 2017/0285045 A1 · Fluorescent probe

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- Number: US 2017/0285045 A1
- Document ID: US20170285045A1
- Status: Published application
- Kind: A1
- Application number: 15/472,414
- Filed: 2017-03-29
- Published: 2017-10-05
- Earliest claimed: 2016-03-31
- Assignees: MUSC Foundation for Research Development
- Applicants: Musc Foundation for Research Development
- Inventors: John J. Lemasters; Andaleb I. Kholmukhamedov; Christopher Lindsey; Craig C. Beeson
- Web page: https://patentgrep.com/patent/US20170285045A1

## CPC

- G01N 33/84: involving inorganic compounds or pH
- A61K 49/0041: {Xanthene dyes, used in vivo, e.g. administered to a mice, e.g. rhodamines, rose Bengal (in vivo G01N)}
- A61K 49/0043: {Fluorescein, used in vivo}
- C09B 11/24: Phthaleins containing amino groups {; Phthalanes; Fluoranes; Phthalides; Rhodamine dyes; Phthaleins having heterocyclic aryl rings; Lactone or lactame forms of triarylmethane dyes}
- G01N 21/6428: {Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53)}
- G01N 33/582: {with fluorescent label}
- G01N 2021/6432: {Quenching}
- G01N 2021/6439: {with indicators, stains, dyes, tags, labels, marks}
- G01N 2800/7009: Oxidative stress
- G01N 2800/7019: Ischaemia
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C09B: ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES {, e.g. PIGMENTS}; MORDANTS; LAKES (fermentation or enzyme using processes to synthesise a desired chemical compound C12P)

## Abstract

Provided herein is a fluorescent probe compound of formula (I): as well as methods of using said compound.

## Claims

### Claim 1

A fluorescent probe compound of formula (I):

### Claim 2 (depends on 1)

A method for detecting the presence or absence of iron in a cell or composition, comprising the steps of: administering the fluorescent probe compound of formula (I) of claim 1 to a cell or composition; exciting said fluorescent probe compound of formula (I); and detecting the presence or absence of emission from said fluorescent probe compound of formula (I), wherein emission from said fluorescent probe compound of formula (I) is quenched by the presence of iron in said cell or composition.

### Claim 3 (depends on 1)

A method for monitoring a cardiovascular or other organ system event, comprising the steps of: administering the fluorescent probe compound of formula (I) of claim 1 to a patient in need thereof; exciting said fluorescent probe compound of formula (I); and detecting the presence or absence of emission from said fluorescent probe compound of formula (I), wherein emission from said fluorescent probe compound of formula (I) is quenched by the presence of iron in said cell or composition.

### Claim 4 (depends on 3)

The method of claim 3, wherein said cardiovascular or organ system event is ischemia.

### Claim 5 (depends on 1)

A kit comprising a fluorescent probe compound of claim 1, and/or instructions for carrying out a method of detecting the presence or absence of iron or copper therewith, optionally in a common package or container.