# US 2017/0285012 A1 · Device for real-time in vivo molecular analysis > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2017/0285012 A1 - Document ID: US20170285012A1 - Status: Published application - Kind: A1 - Application number: 15/512,703 - Filed: 2015-09-22 - Published: 2017-10-05 - Earliest claimed: 2014-09-22 - Assignees: Universite De Sciences Et Technologies De Lille; Centre National De La Recherche Scientifique - Applicants: Universite Des Sciences Et Technologies De Lille; Centre National De La Recherche Scientifique - Inventors: Michel SALZET; Isabelle FOURNIER; Cristian FOCSA; Michael ZISKIND; Benoit FATOU; Maxence WISZTORSKI - Web page: https://patentgrep.com/patent/US20170285012A1 ## CPC - G01N 33/5091: {for testing the pathological state of an organism} - A61B 5/14503: {invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors (A61B5/1459, A61B5/1464, A61B5/1473, A61B5/1482, A61B5/14865 take precedence)} - A61B 5/14546: {for measuring analytes not otherwise provided for, e.g. ions, cytochromes} - A61B 10/02: Instruments for taking cell samples or for biopsy {(A61B10/0038 and A61B10/0045 take precedence; needle locating or guiding means A61B17/3403; samplers for enzymology or microbiology C12M1/26; sampling or preparing biological specimens G01N33/48)} - C12Q 1/02: involving viable microorganisms - G01N 33/566: using specific carrier or receptor proteins as ligand binding reagents {where possible specific carrier or receptor proteins are classified with their target compounds} - H01J 49/0463: {Desorption by laser or particle beam, followed by ionisation as a separate step (sample holder per se H01J49/0418)} - A61B 5/00: Measuring for diagnostic purposes (radiation diagnosis A61B6/00; diagnosis by ultrasonic, sonic or infrasonic waves A61B8/00); Identification of persons - A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) - A61K 49/00: Preparations for testing in vivo - C12N 5/00: Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor; (plant reproduction by tissue culture techniques A01H4/00) - G01N 2001/045: {Laser ablation; Microwave vaporisation} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D) - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - H01J: ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS (spark-gaps H01T; electric arc lamps H05B31/00; particle accelerators H05H) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract The invention relates to a biological material molecular analysis device characterized in that it includes:—a laser (34) optionally using an optical parametric oscillator (OPO), configured to emit a wavelength between 2.5 ym and 12 put, said configured laser (34) being intended to ablate said biological material by ejecting charged and/or uncharged particles;—a mass spectrometer (31); and—a probe (S, 10) comprising at least one first analysis fiber (A, 14), connected to the laser (34), and a transfer tube (T, 21), connected to the mass spectrometer (31). ## Claims ### Claim 1 A molecular analysis device for a biological material comprising: a laser (34), optionally assisted with an optical parametric oscillator (OPO), configured for emitting a wavelength comprised between 2.5 μm and 12 μm, said thereby configured laser (34) being intended to ablate said biological material by ejecting charged and/or non-charged particles; a mass spectrometer (31); and a probe (S, 10) including: at least one first analysis fiber (A, 14) connected to the laser (34), and a transfer tube (T, 21) connected to the mass spectrometer (31). ### Claim 2 (depends on 1) The device according to claim 1, characterized in wherein the laser (34), optionally assisted with an optical parametric oscillator (OPO) is configured for emitting a wavelength comprised between 2.5 μm and 3.5 μm. ### Claim 3 (depends on 1) The device according to claim 1, wherein the laser (34), optionally assisted with an optical parametric oscillator (OPO), is configured for emitting a wavelength comprised between 2.8 μm and 3.2 μm. ### Claim 4 (depends on 1) The device according to claim 1, wherein the laser (34) is a pulsed laser configured for generating a beam for which the energy is comprised between 2 mJ/pulse and 15 mJ/pulse and the surface of which is comprised between 30 μm2 and 3 mm2. ### Claim 5 (depends on 1) The device according to claim 1, wherein the laser (34) is a pulsed laser configured for generating a beam for which the energy is comprised between 5 mJ/pulse and 12 mJ/pulse, for example between 5 mJ/pulse and 10 mJ/pulse and the surface is comprised between 30 μm2 and 3 mm2. ### Claim 6 (depends on 4) The device according to claim 4, wherein the laser (34) is a configured for generating a beam for which the surface area is comprised between 0.5 mm2 and 2 mm2. ### Claim 7 (depends on 1) The device according to claim 1, wherein a system (42) for focussing and/or transferring, molecular ions intended to be formed during ablation of biological material, is interposed between said transfer tube (T, 21) and the mass spectrometer (31). ### Claim 8 (depends on 7) The device according to claim 7, wherein said focussing and/or transfer system (42) is a transfer capillary. ### Claim 9 (depends on 1) The device according to claim 1, wherein said transfer tube (T, 21) is provided with a heating means (44). ### Claim 10 (depends on 6) The device according to claim 6, further comprising a de-pressurization capillary (41) inserted between said transfer tube (T, 21) and said focussing and/or transfer system (42), the inner diameter of this de-pressurization capillary (41) being less than that of the transfer tube (T, 21). ### Claim 11 (depends on 10) The device according to claim 10, wherein the de-pressurization capillary (41) is a metal capillary and in that a means is provided for generating a potential difference between said de-pressurization capillary (41) and the mass spectrometer (31). ### Claim 12 (depends on 1) The device according to claim 1, wherein a metal grid (48) is introduced between said transfer tube (T, 21) and said mass spectrometer (31). ### Claim 13 (depends on 10) The device according to claim 10, further comprising an electrospray capillary (45) connected to said de-pressurization capillary (41) and further connected to a means for distributing solvent (46). ### Claim 14 (depends on 1) The device according to claim 1, is no electrospray capillary connected to a means for distributing solvent is provided between the transfer tube (T, 21) and the mass spectrometer (31). ### Claim 15 (depends on 10) The device according to claim 10, further comprising a distribution capillary connected to said de-pressurization capillary and further connected to a gas distributor. ### Claim 16 (depends on 1) The device according to claim 1, is no distribution capillary connected to a gas distributor is provided between the transfer tube (T, 21) and the mass spectrometer (31). ### Claim 17 (depends on 1) The device according to claim 1, wherein said probe (S, 10) includes a second analysis fiber (15). ### Claim 18 (depends on 1) The device according to claim 1, wherein said probe (S, 10) further includes a therapy fiber (16). ### Claim 19 (depends on 18) The device according to claim 18, wherein said therapy fiber (16) is connected to a therapy laser (36). ### Claim 20 (depends on 1) The device according to claim 1, wherein said probe (S, 10) further includes an illumination channel (12) and an image shooting channel (13).