Methods for Increasing the Stability of Production of Compounds in Microbial Host Cells
AssigneeAlgenol Biotech LLC
InventorsMing-De DENG, Ulf DÜHRING, Frank ULICZKA, Kimberly Lynn ANDERSON
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Abstract
Methods for increasing the genetic stability of genetically enhanced microbial host cells capable of producing a compound of interest are disclosed.
Claims21
A non-naturally occurring auxotrophic microbe capable of production of a compound of interest via the expression of at least one exogenous gene wherein the microbe contains an extrachromosomal plasmid comprising the at least one exogenous gene and a gene whose expression complements the auxotrophy of the non-naturally occurring auxotrophic microbe and wherein the at least one exogenous gene and the gene whose expression complements auxotrophy form a polycistronic operon wherein the at least one exogenous gene and the gene whose expression complements auxotrophy are both operably linked to a promoter such that expression of the gene whose expression complements auxotrophy cannot occur without expression of the at least one exogenous gene.
A non-naturally occurring auxotrophic microbe capable of production of a compound of interest via the expression of at least one exogenous gene wherein the microbe contains a chromosomal polynucleotide sequence comprising the at least one exogenous gene and a gene whose expression complements the auxotrophy of the non-naturally occurring auxotrophic microbe and wherein the at least one exogenous gene and the gene whose expression complements auxotrophy form a polycistronic operon wherein the at least one exogenous gene and the gene whose expression complements auxotrophy are both operably linked to a promoter such that expression of the gene whose expression complements auxotrophy cannot occur without expression of the at least one exogenous gene.
Dependencies are inferred from the claim text.