# US 2017/0283780 A1 · Fatty acid desaturases from primula

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- Number: US 2017/0283780 A1
- Document ID: US20170283780A1
- Status: Published application
- Kind: A1
- Application number: 15/497,058
- Filed: 2017-04-25
- Published: 2017-10-05
- Earliest claimed: 2003-08-21
- Assignees: Monsanto Technology LLC
- Applicants: Monsanto Technology LLC
- Inventors: Virginia Ursin; Fenggao Dong; Byron Froman; Thomas J. La Rosa; Steven E. Screen; Jennifer C. Simmons
- Web page: https://patentgrep.com/patent/US20170283780A1

## CPC

- C12N 9/0004: {Oxidoreductases (1.)}
- A61P 3/02: Nutrients, e.g. vitamins, minerals
- A61P 9/00: Drugs for disorders of the cardiovascular system
- A61P 37/00: Drugs for immunological or allergic disorders
- C12N 15/8247: {involving modified lipid metabolism, e.g. seed oil composition}
- C12P 7/6427: Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}

## Abstract

The invention relates generally to methods and compositions concerning desaturase enzymes that modulate the number and location of double bonds in long chain poly-unsaturated fatty acids (LC-PUFA's). In particular, the invention relates to methods and compositions for improving omega-3 fatty acid profiles in plant products and parts using desaturase enzymes and nucleic acids encoding for such enzymes. In particular embodiments, the desaturase enzymes are Primula Δ6-desaturases. Also provided are improved soybean oil compositions having SDA and a beneficial overall content of omega-3 fatty acids relative to omega-6 fatty acids.

## Claims

### Claim at position 1

1-52. (canceled)

### Claim 53

A method of topical application of SDA comprising providing a lotion or ointment comprising an endogenous soybean oil that comprises SDA and GLA in a ratio of at least 1.3.

### Claim 54 (depends on 53)

The method of claim 53, wherein said endogenous soybean oil further comprises GLA.

### Claim 55 (depends on 54)

The method of claim 54, wherein said ratio of SDA/GLA is at least about 1.5.

### Claim 56 (depends on 55)

The method of claim 55, wherein said ratio of SDA/GLA is at least about 2.0.

### Claim 57 (depends on 56)

The method of claim 56, wherein said ratio of SDA/GLA is at least about 2.5.

### Claim 58 (depends on 53)

The method of claim 53, wherein said oil further comprises ALA.

### Claim 59 (depends on 58)

The method of claim 58, further comprising a ratio of SDA/ALA wherein said ratio of SDA/ALA is at least about 0.5.

### Claim 60 (depends on 59)

The method of claim 59, wherein said ratio of SDA/ALA is at least about 1.0.

### Claim 61 (depends on 60)

The method of claim 60, wherein said ratio of SDA/ALA is at least about 1.5.

### Claim 62 (depends on 61)

The method of claim 61, wherein said ratio of SDA/ALA is less than about 1.8.

### Claim 63 (depends on 53)

The method of claim 53, wherein said topically applying comprises applying to skin.

### Claim 64 (depends on 53)

The method of claim 53, wherein said lotion further comprises an excipient.

### Claim 65 (depends on 53)

The method of claim 53, wherein said SDA comprises SDA in a form selected from the group consisting of conjugated forms, esters, amides, prodrugs of the fatty acids, sodium salts, potassium salts, lithium salts, N-alkylpolyhydroxamine salts, N-methyl glucamine, and ethyl esters.