# US 2017/0283449 A1 · Stevia extracts

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- Number: US 2017/0283449 A1
- Document ID: US20170283449A1
- Status: Published application
- Kind: A1
- Application number: 15/507,969
- Filed: 2015-08-27
- Published: 2017-10-05
- Earliest claimed: 2014-09-02
- Assignees: PureCircle Sdn Bhd; The Coca-Cola Company
- Applicants: PureCircle Sdn Bhd; The Coca-Cola Company
- Inventors: Avetik MARKOSYAN; Indra PRAKASH
- Web page: https://patentgrep.com/patent/US20170283449A1

## CPC

- A23L 2/60: Sweeteners
- A01H 1/02: Methods or apparatus for hybridisation; Artificial pollination {; Fertility}
- A01H 4/005: {Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques}
- A01H 4/008: {Methods for regeneration to complete plants}
- A01H 5/12: Leaves
- A01H 6/1488: {Stevia}
- A21D 2/181: {Sugars or sugar alcohols (honey A21D2/34)}
- A21D 2/36: Vegetable material
- A21D 13/062: with modified sugar content; Sugar-free products
- A23C 9/1307: {Milk products or derivatives; Fruit or vegetable juices; Sugars, sugar alcohols, sweeteners; Oligosaccharides; Organic acids or salts thereof or acidifying agents; Flavours, dyes or pigments; Inert or aerosol gases; Carbonation methods}
- A23G 3/36: characterised by the composition {containing organic or inorganic compounds}
- A23G 4/06: characterised by the composition {containing organic or inorganic compounds}
- A23L 2/06: from citrus fruits
- A23L 2/52: Adding ingredients (adding preservatives A23B70/10)
- A23L 2/54: Mixing with gases
- A23L 27/36: {Terpene glycosides}
- A23L 33/105: Plant extracts, their artificial duplicates or their derivatives
- A61K 8/9789: Magnoliopsida [dicotyledons]
- A61Q 1/00: Make-up preparations; Body powders; Preparations for removing make-up
- A61Q 11/00: Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
- C07H 1/08: from natural products
- C07H 15/24: Condensed ring systems having three or more rings
- C07H 15/256: Polyterpene radicals
- C12N 15/8282: {for fungal resistance}
- A23G 3/42: characterised by the carbohydrates used, e.g. polysaccharides (containing dairy products A23G3/46)
- A23G 4/10: characterised by the carbohydrates used, e.g. polysaccharides (containing dairy products A23G4/16)
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- A23V 2250/262: Stevioside
- A61K 36/00: Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines {(antigens from pollen A61K39/36)}
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A21D: TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS (preservation of flour or dough before baking A23B40/00; preservation of bakery products A23B45/00)
- A23C: DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF (preservation thereof A23B11/00)
- A23G: COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- C07H: SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS (derivatives of aldonic or saccharic acids C07C, C07D; aldonic acids, saccharic acids C07C59/105, C07C59/285; cyanohydrins C07C255/16; glycals C07D; compounds of unknown constitution C07G; polysaccharides, derivatives thereof C08B; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; sugar industry C13)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION

## Abstract

A Stevia extract made from leaves of the Stevia rebaudiana plant is described. The extract has desired levels of steviol glycosides and is useful in food, beverage, and other consumable products.

## Claims

### Claim 1

A process for preparing steviol glycoside compositions, comprising the steps of:
a) providing Stevia rebaudiana leaves, wherein the Stevia rebaudiana leaves comprise RebM in an amount above a common relative concentration of RebM;
b) providing a solvent comprising water,
c) contacting the Stevia rebaudiana leaves with solvent to extract the steviol glycosides from the leaves; and
d) separating the Stevia rebaudiana leaves to obtain Stevia extract solution comprising RebM, wherein the RebM is present in the solution above its common relative concentration;
and wherein for RebM the common relative concentration is 1.4%.

### Claim 2 (depends on 1)

The process of claim 1 further comprising purification of the Stevia extract solution and drying to obtain Stevia extract with total steviol glycoside content of at least 6.9% (wt/wt on dry basis).

### Claim 3 (depends on 1)

The process of claim 1 wherein Stevia rebaudiana leaves are obtained from at least one Stevia rebaudiana cultivar selected from the group consisting of: “807086” wherein a representative sample of live plant tissue of said “807086” cultivar has been deposited under CGMCC No. 9702; “814011” wherein a representative sample of live plant tissue of said “814011” cultivar has been deposited under CGMCC No. 9701; and “817096” wherein a representative sample of live plant tissue of said “817096” cultivar has been deposited under CGMCC No. 9703.

### Claim 4 (depends on 1)

The process of claim 1 wherein Stevia rebaudiana leaves are obtained from a cultivar generated using at least one Stevia rebaudiana cultivar selected from group consisting of: “807086” wherein a representative sample of live plant tissue of said “807086” cultivar has been deposited under CGMCC No. 9702; “814011” wherein a representative sample of live plant tissue of said “814011” cultivar has been deposited under CGMCC No. 9701; and “817096” wherein a representative sample of live plant tissue of said “817096” cultivar has been deposited under CGMCC No. 9703.

### Claim 5 (depends on 1)

An untreated aqueous Stevia extract prepared by the process of claim 1, wherein RebM is present in the extract at or above its common relative concentration.

### Claim 6 (depends on 5)

A food, beverage or other consumable comprising Stevia extract of claim 5.