# US 2017/0282154 A1 · Sorbent material for separating bio-macromolecules

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- Number: US 2017/0282154 A1
- Document ID: US20170282154A1
- Status: Published application
- Kind: A1
- Application number: 15/510,229
- Filed: 2015-09-10
- Published: 2017-10-05
- Earliest claimed: 2014-09-10
- Assignees: Quantumdx Group Limited
- Applicants: QuantuMDx Group Limited
- Inventors: Jonathan James O'HALLORAN; Joseph H. HEDLEY; Sam WHITEHOUSE; Marc FLANAGAN; John TYSON; Rachael DIXON; Sam BHATT; Emily CROZIER; Chris HOWELL
- Web page: https://patentgrep.com/patent/US20170282154A1

## CPC

- B01J 20/261: {obtained by reactions only involving carbon to carbon unsaturated bonds (macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds per se C08F)}
- B01J 20/103: {comprising silica}
- B01J 20/28004: {Sorbent size or size distribution, e.g. particle size}
- B01J 20/28016: {Particle form}
- B01J 20/28023: {Fibres or filaments (fibres or filaments in the form of membranes B01J20/28038; B01J20/28007 takes precedence)}
- B01J 20/28026: {Particles within, immobilised, dispersed, entrapped in or on a matrix, e.g. a resin}
- B01J 20/28033: {Membrane, sheet, cloth, pad, lamellar or mat}
- B01J 20/28042: {Shaped bodies; Monolithic structures}
- B01J 20/28059: {being less than 100 m2/g}
- B01J 20/28061: {being in the range 100-500 m2/g}
- B01J 20/2808: {being less than 2 nm, i.e. micropores or nanopores}
- B01J 20/28083: {being in the range 2-50 nm, i.e. mesopores}
- B01J 20/28085: {being more than 50 nm, i.e. macropores}
- B01J 20/286: Phases chemically bonded to a substrate, e.g. to silica or to polymers
- B01J 20/3204: {Inorganic carriers, supports or substrates}
- B01J 20/327: {Polymers obtained by reactions involving only carbon to carbon unsaturated bonds}
- B01J 20/3276: {Copolymers}
- C12N 15/1006: {by means of a solid support carrier, e.g. particles, polymers}
- G01N 1/405: {by adsorption or absorption}
- B01J 2220/54: Sorbents specially adapted for analytical or investigative chromatography
- B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A sorbent material is disclosed for the one-step separation of bio-macromolecules in a single pass extraction of DNA from complex mixtures of molecules and chemicals. In one embodiment, the sorbent material comprises a silanized material at least partially coated or formed with a polymer selected from the group consisting of a poly(aryl methacrylate), a poly(aryl acrylate), a poly(heteroaryl methacrylate, a poly(heteroaryl acrylate) and a copolymer thereof.

## Claims

### Claim 1

A sorbent material for separating bio-macromolecules, the sorbent material comprising a silanized material at least partially coated or formed with a polymer selected from the group consisting of a poly(aryl methacrylate), a poly(aryl acrylate), a poly(heteroaryl methacrylate, a poly(heteroaryl acrylate) and a copolymer thereof.

### Claim 2 (depends on 1)

The sorbent material of claim 1, wherein the silanized material is an inorganic material selected from the group consisting of a silanized silica particle, a silanized silica fiber and a silanized silica monolithic membrane or structure.

### Claim 3 (depends on 1)

The sorbent material of claim 1, wherein the silanized material is an organic material selected from the group consisting of porous organic materials and membranes.

### Claim 4 (depends on 1)

The sorbent material of claim 1, wherein the polymer comprises a recurring unit selected from the group consisting of anisolemethyl methacrylate, phenylethanol methacrylate, pyridinemethyl methacrylate and naphthalenemethyl methacrylate.

### Claim 5 (depends on 1)

The sorbent material of claim 1, wherein the sorbent material has a wettability value in the range of about 65° to about 100°.

### Claim 6 (depends on 1)

The sorbent material of claim 1, wherein the sorbent material has a surface area in the range of about 0.1 m2 to about 130 m2.

### Claim 7 (depends on 1)

The sorbent material of claim 1, wherein the silanized inorganic material comprises silanized silica particles having an average pore size in the range of about 1 nm to about 100 nm.

### Claim 8

(canceled)

### Claim 9 (depends on 1)

The sorbent material of claim 1, wherein the silanized inorganic material comprises silanized silica particles having an average diameter of less than about 200 microns.

### Claim 10

(canceled)

### Claim 11 (depends on 1)

An article comprising the sorbent material of claim 1, wherein the article is in a form selected from particulate, monolithic or membrane-like, and wherein an average interstitial distance is greater than about 10 nm.

### Claim 12 (depends on 11)

The article of claim 11, wherein the average interstitial distance is less than about 12 microns.

### Claim 13 (depends on 12)

The article of claim 12, wherein the article is in the form of a membrane, and wherein the sorbent material is embedded in a porous organic or inorganic matrix.

### Claim 14

A method of making a sorbent material comprising silanized silica at least partially coated with poly(benzyl methacrylate), the method comprising:
suspending silica in a solution of dimethylvinylchlorosilane in trifluorotoluene;
removing the liquid and resuspending the silica in a fresh solution of dimethylvinylchlorosilane in trifluorotoluene;
optionally removing the liquid and resuspending the silica again in a fresh 5% solution of dimethylvinylchlorosilane in trifluorotoluene;
collecting and drying the resulting silanized silica;
adding the silanized silica, benzylmethacrylate and potassium peroxodisulfateto a stirred solution of sodium stearate in water; and
collecting and drying the resultant sorbent material comprising silanized silica coated with poly(benzyl methacrylate).

### Claim 15

A dual processing device for lysing a biological sample and isolating nucleic acids from the biological sample, the device comprising:
a sample input unit;
a sample lysis unit;
a nucleic acid extraction unit; and
a nucleic acid reception unit;
wherein the nucleic acid extraction unit comprises a silanized material at least partially coated or formed with a polymer selected from the group consisting of a poly(aryl methacrylate), a poly(aryl acrylate), a poly(heteroaryl methacrylate, a poly(heteroaryl acrylate) and a copolymer thereof, wherein the silanzied material is configured to retain proteins and other non-nucleic acids from the biological sample and allow the nucleic acids to pass through into the nucleic acid reception unit.

### Claim 16 (depends on 15)

The device according to claim 15, wherein the sample lysis unit comprises a blade that is configured to rotate within the sample lysis unit so as to lyse the biological sample.

### Claim 17 (depends on 15)

The device according to claim 15, wherein the sample lysis unit comprises beads.

### Claim 18 (depends on 16)

The device according to claim 16, wherein the device further comprises a spring loaded plunger that is configured to control rotation of the blade in the sample lysis unit.

### Claim 19 (depends on 15)

The device according to claim 15, wherein the device further comprises a buffer that is configured to act as a mobility carrier when the biological sample is processed in the sample lysis unit and/or the nucleic acid extraction unit.

### Claim 20 (depends on 15)

The device according to claim 15, wherein the nucleic acid reception unit comprises a cap.

### Claim 21 (depends on 20)

The device according to claim 20, wherein the cap is a pressure sealed cap that can be removed from the device.

### Claim 22

A sorbent material for separating bio-macromolecules, the sorbent material comprising a silanized material at least partially coated or formed with a polymer, wherein the sorbent material comprises an average interstitial spacing of greater than about 6 um and less than about 23 um, a pore size of greater than about 10 nm and less than about 200 nm, and a surface area of greater than about 0.1 m2 and not more than about 150 m2.