# US 2017/0280760 A1 · Method for the production of a porous powder product containing a probiotic or other microorganisms > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2017/0280760 A1 - Document ID: US20170280760A1 - Status: Published application - Kind: A1 - Application number: 15/630,622 - Filed: 2017-06-22 - Published: 2017-10-05 - Earliest claimed: 2012-06-13 - Assignees: Clextral - Applicants: Clextral - Inventors: Maxime Collado; Jean-Marie Bouvier - Web page: https://patentgrep.com/patent/US20170280760A1 ## CPC - A23L 33/135: Bacteria or derivatives thereof, e.g. probiotics - A23P 10/30: Encapsulation of particles, e.g. foodstuff additives - A23P 10/40: free-flowing powder or instant powder, i.e. powder which is reconstituted rapidly when liquid is added (A23P10/20, A23P10/30 take precedence) - A23P 30/40: Foaming or whipping - C12N 1/04: Preserving or maintaining viable microorganisms (immobilised microorganisms C12N11/00) - A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs - A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B) - A23P: SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION ## Abstract The invention proposes a method for the production of a porous powder product containing a microorganism, from a liquid concentrate comprising at least one liquid microorganism culture and a carrier composition, the carrier composition comprising at least one carbohydrate and at least one protein, the method comprising the steps of: i) preparing the liquid concentrate to have both a temperature of between and 40° C. and a viscosity greater than 150 mPa·s; ii) injecting a food grade gas into the prepared concentrate at a pressure greater than 0.3 MPa to intimately incorporate the gas into the liquid concentrate and form a stable foam with a density of between about 0.6 to about 1.2 kg/l; iii) conveying the foam to an atomizing device wherein the pressure of the foam is reduced from 0.3 MPa to atmospheric pressure to provide a partially dried product; and iv) exposing the partially dried product to a drying media to reduce both the moisture content to less than 5% moisture and the water activity to less than 0.25 Aw, when measured at 20° C., and to provide the porous powder product, wherein the temperature of the porous powder product in step (iv) does not exceed 50° C. ## Claims ### Claim 1 A method for the production of a porous powder product containing a microorganism, from a liquid concentrate comprising at least one liquid microorganism culture and a carrier composition, the carrier composition comprising at least one carbohydrate and at least one protein, the method comprising the steps of: i) preparing the liquid concentrate both to have a temperature of between 5 and 40° C. and to have, at the temperature of the prepared liquid concentrate, a viscosity greater than 150 mPa·s; ii) incorporating a pressurized food grade gas into the prepared liquid concentrate at a pressure greater than 0.3 MPa, step (ii) being conducted in a hermetically sealed tank containing the liquid concentrate, step (ii) including injecting the pressurized gas into the liquid concentrate in the hermetically sealed tank then shaking the hermetically sealed tank so as to mix the liquid concentrate with the injected gas effective to form a stable foam having a density of between about 0.6 to about 1.2 kg/l; iii) conveying the stable foam to an atomizing device in which the pressure of the stable foam is reduced from 0.3 MPa to atmospheric pressure to release the gas from the stable foam for providing a partially dried product; and iv) exposing the partially dried product to a drying media to reduce both the moisture content to less than 5% moisture and the water activity to less than 0.25 Aw, when measured at 20° C., and to provide the porous powder product, wherein the temperature of the porous powder product in step (iv) does not exceed 50° C. ### Claim 2 (depends on 1) The method according to claim 1, wherein the temperature of the prepared liquid concentrate is from 25 to 35° C. ### Claim 3 (depends on 1) The method according to claim 1, wherein the density of the stable foam is between 0.8 and 1.1 kg/l. ### Claim 4 (depends on 1) The method according to claim 1, wherein the food grade gas is selected from the group comprising carbon dioxide, oxygen, argon, nitrogen, nitrogen dioxide, air, or mixtures thereof. ### Claim 5 (depends on 1) The method according to claim 1, wherein the carrier composition comprises maltodextrin, sugars having glass transition temperature greater than 55° C., such as lactose, maltose and sucrose, water soluble gums such as acacia, guar or xanthan, hydroxypropylmethylcellulose (HPMC), alginates, pectin, milk powders, carbohydrates, proteins, or mixtures thereof, in order to aid the liquid culture concentrate to foam and to dissolve gas. ### Claim 6 (depends on 1) The method according to claim 1, wherein step (iv) comprises a first substep in which the partially dried product is discharged into a drying chamber. ### Claim 7 (depends on 6) The method according to claim 6, wherein step (iv) comprises further a second substep, which is subsequent to the first substep and which is conducted on a fluid bed. ### Claim 8 (depends on 1) The method according to claim 1, wherein, in step (iv), the water activity of the partially dried product is reduced to between 0.1 and 0.25 Aw, when measured at 20° C. ### Claim 9 (depends on 1) The method according to claim 1, wherein the bulk density of the porous powder product is between 0.2 g/cm3 and 0.6 g/cm3. ### Claim 10 (depends on 1) The method according to claim 1, wherein the porous powder product is a free-flowing powder, with a particles size between 30 μm to 200 μm. ### Claim 11 (depends on 1) The method according to claim 1, wherein the microorganism is selected from the group comprising bacteria, yeast, fungi and probiotics. ### Claim at position 12 12-13. (canceled) ### Claim 14 (depends on 1) The method according to claim 1, wherein step (i) is conducted in a thermomechanical treatment device. ### Claim 15 (depends on 1) The method according to claim 1, wherein step (iv) is followed by agglomeration. ### Claim 16 (depends on 8) The method according to claim 8, wherein, in step (iv), the water activity of the partially dried product is reduced to around 0.15 Aw, when measured at 20° C. ### Claim 17 (depends on 9) The method according to claim 9, wherein the bulk density of the porous powder product is around 0.3 g/cm3. ### Claim 18 (depends on 14) The method according to claim 14, wherein the thermomechanical treatment device is selected from the group comprising a single screw extruder, twin screw extruder, votators, and scrape surface heat exchangers. ### Claim 19 (depends on 1) The method according to claim 1, wherein step (ii) is further conducted in an aeration device, and wherein after being mixed by shaking in the hermetically sealed tank, the gas and the liquid concentrate are further mixed in the aeration device. ### Claim 20 (depends on 1) The method according to claim 1, wherein step (iii) includes connecting an exit line of the hermetically sealed tank to the atomizing device. ### Claim 21 (depends on 2) The method according to claim 2, wherein the temperature of the prepared concentrate is about 30° C. ### Claim 22 (depends on 3) The method according to claim 3, wherein the density is around 0.85 kg/l.