# US 2017/0280730 A1 · Compositions and methods useful for the prevention of malaria and dengue virus transmission

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- Number: US 2017/0280730 A1
- Document ID: US20170280730A1
- Status: Published application
- Kind: A1
- Application number: 15/507,332
- Filed: 2015-08-28
- Published: 2017-10-05
- Earliest claimed: 2014-08-28
- Assignees: The Johns Hopkins University
- Applicants: The Johns Hopkins University
- Inventors: George Dimopoulos; Sarah M. Short; Jose L. Ramirez
- Web page: https://patentgrep.com/patent/US20170280730A1

## CPC

- A01N 63/20: Bacteria; Substances produced thereby or obtained therefrom
- A61K 31/7004: Monosaccharides having only carbon, hydrogen and oxygen atoms
- A61K 35/74: Bacteria (therapeutic use of a bacterial protein A61K38/00)
- C12N 1/20: Bacteria; Culture media therefor
- C12N 1/205: Bacterial isolates
- A61K 2300/00: Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
- C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales}
- Y02A 50/30: Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

The present invention relates to the fields of malaria and dengue virus. More specifically, the present invention provides compositions and methods useful for the treatment and prevention of malaria and dengue virus. In particular embodiments, a composition comprises mosquito nectar feed and Chromobacterium sp_Panamam (Csp_P).

## Claims

### Claim 1

A composition comprising mosquito nectar feed and Chromobacterium sp_Panamam (Csp_P).

### Claim 2 (depends on 1)

The composition of claim 1, wherein the Csp-P is comprises a biofilm.

### Claim 3 (depends on 1)

The composition of claim 1, wherein the biofilm is fresh or desiccated.

### Claim 4 (depends on 1)

The composition of claim 1, wherein the Csp-P is comprises a culture.

### Claim 5 (depends on 1)

The composition of claim 1, wherein the Csp_P comprises a supernatant.

### Claim 6 (depends on 1)

The composition of claim 1, wherein the Csp_P comprises a filtrate.

### Claim 7 (depends on 1)

The composition of claim 1, wherein the Csp_P has the 16s rDNA gene sequence of SEQ ID NO:1.

### Claim 8 (depends on 1)

The composition of claim 1, wherein the nectar feed comprises one or more of sucrose, dextrose and fructose.

### Claim 9 (depends on 1)

A method for controlling malaria and dengue virus transmission via mosquitoes comprising applying the composition of claim 1 in an area where the mosquitoes are to be controlled.

### Claim 10 (depends on 9)

The method of claim 9, wherein the mosquitoes comprise Anopheles and Aedes mosquitoes.

### Claim 11 (depends on 10)

The method of claim 10, wherein the Anopheles mosquitoes comprise Anopheles gambiae mosquitoes.

### Claim 12 (depends on 10)

The method of claim 10, wherein the Aedes mosquitoes comprise Aedes aegypti mosquitoes.

### Claim 13

A composition comprising a biofilm, supernatant, filtrate or extract of a biologically pure culture of Chromobacerium sp. (Csp_P).

### Claim 14 (depends on 13)

The composition of claim 13, wherein the biologically pure culture of Csp_P has the 16S rDNA gene sequence of SEQ ID NO:1.

### Claim 15

A method for controlling Anopheles and Aedes mosquitoes comprising applying in an area where the mosquitoes are to be controlled a composition comprising an effective insect control amount of a supernatant, filtrate or extract of a biologically pure culture of Csp_P.

### Claim 16 (depends on 15)

The method of claim 15, wherein the composition further comprise a sugar source.

### Claim 17 (depends on 16)

The method of claim 16, wherein the sugar comprises sucrose, dextrose and/or fructose.

### Claim 18 (depends on 1)

The method of claim 1, wherein the Csp_P is the bacteria having the characteristics of ATCC Designation No. PTA-121570.

### Claim 19 (depends on 9)

The method of claim 9, wherein the Csp_P is the bacteria having the characteristics of ATCC Designation No. PTA-121570.

### Claim 20 (depends on 15)

The method of claim 15, wherein the Csp_P is the bacteria having the characteristics of ATCC Designation No. PTA-121570.